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目的：寻找有效的多药耐药联合逆转剂。方法：用ＭＴＴ法、联合效应分析和流式细胞仪测定技术研究奎宁（Ｑｕｉｎ）分别与环孢菌素Ａ（ＣｓＡ）、他莫西酚（Ｔａｍ）、潘生丁（ＤＰＭ）联合逆转耐药细胞系Ｋ５６２／ＨＨＴ对柔红霉素（ＤＮＲ）和高三尖杉酯碱（ＨＨＴ）耐药。结果：逆转倍数是单用药的２～３倍，达到或超过单用２倍剂量的逆转效果，逆转剂间有联合协同作用，其中Ｑｕｉｎ与ＤＰＭ或Ｔａｍ联合的协同作用大于Ｑｕｉｎ与ＣｓＡ联合，可增加细胞内ＤＮＲ浓度。结论：Ｑｕｉｎ与ＣｓＡ、ＤＰＭ或Ｔａｍ联合具有协同作用，不同机制逆转剂联合的协同作用更为显著。
Objective: To find an effective multidrug resistance combination reversal agent. METHODS: MTT assay, combined effect analysis, and flow cytometry techniques were used to study the combination of quinine and cyclosporin A (CsA), tamoxifen (Tam), and dipyridamole (DPM) in reversing drug-resistant cells. The K562/HHT was resistant to daunorubicin (DNR) and homoharringtonine (HHT). Results: The reversal multiples were 2 to 3 times that of single use, which achieved or exceeded the reversal effect of a single dose of 2 doses. There was a synergistic effect between the reversal agents. The synergistic effect of Quin with DPM or Tam was greater than that of Quin with CsA. Increase the intracellular DNR concentration. CONCLUSION: Quin and CsA, DPM, or Tam have a synergistic effect. The synergistic effect of the combination of different mechanism reversal agents is more significant.