论文部分内容阅读
目的对比研究肺炎双球菌及博莱霉素(BLM)所致肺泡炎的影像学表现及其病理对照,探讨实验性肺纤维化影像学变化特征及其病理基础。方法选用SD大鼠90只,随机分为BLM组、肺炎组及正常参照组。分别采用博莱霉素、肺炎双球菌及生理盐水气管内一次灌注的方法制作大鼠肺损伤模型及正常参照模型,分时间点将大鼠麻醉后行高分辨率CT(HRCT)扫描,随即取动物肺组织制作成标本,HE染色观察。结果肺炎及BLM性肺泡炎第1天组影像学表现均为双肺野透光度减低。BLM第3天组可见以主支气管为中心呈放射状分布的高密度影。第5~7天组肺泡炎表现较前减弱,同时在肺内、中带及心缘旁出现纤维索条影。肺炎第3~7天组影像学表现为双肺散在的淡薄云雾影。对照病理观察,此阶段2组大鼠肺泡及肺间质中均有大量中性粒细胞及巨噬细胞浸润,其中肺炎组大鼠肺泡腔内炎性渗出液较多;而BLM组大鼠肺泡腔内以细胞浸润为主,且BLM组大鼠肺出血、实变较肺炎组严重。第5~7天组,BLM组大鼠肺间质中还可见成纤维细胞增生。结论BLM所致肺泡炎影像学表现与肺炎双球菌肺泡炎影像学表现具有一些不同的特点。
Objective To compare the imaging manifestations and pathological changes of alveolitis caused by Streptococcus pneumoniae and bleomycin (BLM), and to explore the characteristics of pathological changes and pathological changes of experimental pulmonary fibrosis. Methods 90 SD rats were randomly divided into BLM group, pneumonia group and normal control group. The model of lung injury and normal control model were made by intraperitoneal injection of bleomycin, pneumococcus and saline respectively. The rats were anesthetized and screened by high resolution CT (HRCT) at different time points. Animal lung tissue made into specimens, HE staining observed. Results On the first day of pneumonia and BLM alveolitis, the imaging performance of both groups was diminished. On day 3 of BLM, a high-density shadow with radial distribution centered on the main bronchus was seen. On the 5th to 7th day, the performance of alveolitis was weakened compared with that of the previous group. Pneumonia 3 to 7 days group imaging performance of the lungs scattered in the light cloud shadow. Compared with the pathological observation, there were a large number of infiltration of neutrophils and macrophages in the alveoli and interstitium of rats in the two groups at this stage, of which pneumonia group had more inflammatory exudate in the alveolar cavity; while BLM rats Alveolar cavities dominated cell infiltration, and BLM rats pulmonary hemorrhage, real change more severe than pneumonia group. On days 5 to 7, fibroblasts were also observed in lung interstitium of BLM rats. Conclusions The manifestations of pulmonary alveolitis due to BLM have some different characteristics from those of pneumococcal alveolitis.