目的建立鼠疫菌脉冲场凝胶电泳的操作规范,并对操作进行风险评估。方法应用疫苗株EV76进行实验,比较不同培养时间、两种酶切的电泳图形,以及胶块中细菌存活情况的检测。结果不同培养时间对电泳结果没有影响,FseI酶切图谱条带较容易分析,胶块内无细菌存活。结论严格执行标准操作程序,才能确保鼠疫菌脉冲场凝胶电泳(PFGE)操作的实验室生物安全。 Objective To establish the operation rules of the gel electrophoresis of Y. pestis pulsed field and evaluate the risk of the operation. Methods The vaccine strain EV76 was used to test the electrophoresis patterns of different culture time, two kinds of enzymes and the detection of the bacterial survival in the plastic block. Results Different incubation time had no effect on the electrophoresis results. The FseI digestion bands were easier to analyze and there was no bacterial in the gel. Conclusion Strict implementation of standard operating procedures in order to ensure that the plague gel electrophoresis (PFGE) operation of laboratory biosafety.