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经接合转移将luxAB基因插入弗氏中华根瘤菌 (Rhizobiumfredii)染色体 ,取可高效结瘤固氮的R .frediilux3菌株用于分子生态学研究 .在不同基模势下 ,研究了lux3在土壤中的存活 ,基模势为 - 30和 - 75 0kPa时 ,lux3在灭菌土中的存活能力明显 (P <0 .0 5 )高于未灭菌土 ,当基模势为 - 15 0 0kPa时 ,土壤是否灭菌对lux3的存活影响没有差异 .不同基模势对lux3活性影响显著 ,在 - 15 0 0kPa的未灭菌土中随着饥饿时间的延长 ,可恢复活性的细菌数量显著下降 .比较了测定微生物活性的 3种方法 (即脱氢酶活性、生物发光和微生物呼吸 ) ,发光值变化与活细胞密度变化一致 .图 4表 2参 8
The luxAB gene was inserted into the chromosome of Rhizobium fredii via conjugation and transfer, and the Rfrediilux3 strain, which can efficiently nodulate and fix nitrogen, was used in the molecular ecology study. The lux3’s survival in the soil was studied under different base modes , The viability of lux3 in sterilized soil was significantly higher (P <0. 05) than that of non-sterilized soil at base pressures of -30 and -75 0 kPa. When the base-mode potential was -1500 kPa, soil There was no difference in the effect of sterilization on the survival of lux3.The effects of different fundamental modes on the lux3 activity were significant, and the number of bacteria that could be restored to active activity decreased significantly with the increase of the starvation time in the non-sterile soil of -150 kPa Three methods for measuring microbial activity (ie, dehydrogenase activity, bioluminescence, and microbial respiration) showed changes in luminescence values consistent with changes in viable cell density Figure 4 Table 2 Reference 8