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在基础培养基中添加14种碳源和11种氮源测定,球孢白僵菌HFW-05菌株的萌发率、生长速率、产孢量及胞外蛋白酶活性。综合各项指标得出,HFW-05菌株生长的最适碳源为蔗糖,产孢量与产酶量最大,分别为16.13×107孢子/cm2和117.38μg/(min.mL);最适氮源为豆粕粉,其产孢量和酶活远高于对照和无机盐类,分别为15.62×107孢子/cm2和74.01μg/(min.mL)。除硝酸钾外,无机盐类在供试浓度上均对孢子萌发和生长有抑制作用。在各种碳(氮)源培养基中添加1%蛋白胨(葡萄糖)后,菌株的产孢量和产酶量均有所提高。结果表明,HFW-05菌株的产孢量和胞外蛋白酶之间具有一定的相关性。
14 kinds of carbon sources and 11 kinds of nitrogen sources were added into basal medium to determine the germination rate, growth rate, sporulation amount and extracellular protease activity of Beauveria bassiana HFW-05 strain. The results showed that the optimal carbon source for the growth of HFW-05 strain was sucrose, with the highest spore yield and enzyme yield of 16.13 × 107 spores / cm2 and 117.38μg / (min.mL), respectively. The optimum nitrogen The source was soybean meal, and its sporulation and enzyme activities were much higher than those of the control and inorganic salts, which were 15.62 × 107 spores / cm2 and 74.01 μg / (min.mL), respectively. In addition to potassium nitrate, the inorganic salts at the test concentration on spore germination and growth inhibition. After adding 1% peptone (glucose) to various kinds of carbon (nitrogen) source medium, the sporulation amount and enzyme production of the strain increased. The results showed that there was a certain correlation between sporulation amount of HFW-05 strain and extracellular protease.