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通过测定 [3 H]胸腺嘧啶核苷 ( [3 H]Td R)参入和结晶紫染色法测定平滑肌细胞增殖 ,研究了溶血磷脂酰胆碱 ( LPC)刺激牛脑微血管平滑肌细胞( BCMSMC)增殖的细胞内信号转导途径 .结果显示 ,LPC能浓度依赖性 ( 1 nmol· L-1- 1 0μmol·L-1)诱导 BCMSMC摄取 [3 H]Td R,在 LPC的浓度为 1 0μmol· L-1时作用达最大 ,cpm由 366± 1 42升至 1 761± 2 96( P<0 .0 1 ) ;LPC亦能浓度依赖性( 1 nmol· L-1- 1 0μmol· L-1)诱导 BCMSMC增殖 ,在 LPC浓度为 1 μmol· L-1时促增殖作用达坪值 ,A595nm由 0 .0 60± 0 .0 0 9增至 0 .1 0 0± 0 .0 1 5( P<0 .0 1 ) .丝裂原激活蛋白激酶 ( MAPK)特异性抑制剂 PD980 59( 2 - 50 μmol· L-1) ,血小板衍生生长因子受体抑制剂酪氨酸磷酸化抑制剂 AG 1 2 96( 2 -50 μmol· L-1)以及蛋白质酪氨酸激酶抑制剂除莠霉素 A( 2 - 1 0μmol· L-1)能浓度依赖性地抑制LPC的上述作用 .表明 LPC能促进 BCMSMC增殖 ,其细胞内信号转导与 MAPK途径有关 .
The proliferation of smooth muscle cells was measured by measuring the incorporation of [3 H] Td R and crystal violet staining, and the proliferation of bovine cerebral microvascular smooth muscle cells (BCMSMC) was stimulated by lysophosphatidylcholine (LPC) Intracellular signal transduction pathway.The results showed that [3 H] Td R uptake by BCG can be induced by LPC in a concentration-dependent manner (1 nmol · L-1 -1 0μmol·L-1) 1 at 1 h, cpm increased from 366 ± 1 42 to 1 761 ± 2 96 (P <0.01), and LPC was also induced in a concentration-dependent manner (1 nmol · L-1 -1 0μmol · L-1) The proliferation of BCMSMC reached the plateau when LPC concentration was 1 μmol · L-1, and A595nm increased from 0.060 ± 0.09 to 0.100 ± 0.015 (P <0 .0 1) PD980 59 (2 - 50 μmol · L-1), a specific inhibitor of mitogen activated protein kinase (MAPK), an inhibitor of tyrosine phosphorylation of platelet-derived growth factor receptor AG 1 2 96 (2 -50 μmol · L-1) and the protein tyrosine kinase inhibitor herbimycin A (2 -1 0μmol · L-1) inhibited the above effects of LPC in a concentration-dependent manner, indicating that LPC can promote the proliferation of BCMSMC , Its intracellular signal transduction and MAPK Diameter about.