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目的通过微弧氧化—硅烷偶联不同浓度抗菌肽KSL对钛片表面进行成骨细胞相容性检测和牙龈卟啉单胞菌抗菌性检测,评价其生物相容性及抗菌性。方法超声微弧氧化-碱处理-硅烷膜层为对照组(A组),载抗菌肽KSL 0.25 mg/ml(B组)、KSL 0.50 mg/ml(C组)、KSL 0.75mg/ml(D组)为实验组。扫描电镜观察试件表面形貌特征,CCK-8检测不同时间段细胞黏附及增殖情况,ALP检测不同时间段碱性磷酸酶活性,激光共聚焦显微镜观察试件表面的活死菌比例数。结果 CCK-8和ALP的检测结果显示四组材料表面的增殖、黏附和碱性磷酸酶活性顺序为D组>C组>B组>A组,四组的比较差异均具有统计学意义,激光共聚焦显微镜显示在有效杀菌浓度下抗菌肽KSL浓度越高抗菌效果越好。结论抗菌肽KSL涂层具有良好的生物相容性与抗菌性。
OBJECTIVE To evaluate the biocompatibility and antimicrobial activity of KSL on the surface of titanium plate by means of micro-arc oxidation-silane coupling KSL with different concentrations of antimicrobial peptides and the antimicrobial activity of Porphyromonas gingivalis. Methods Ultrasound microarc oxidation - alkali treatment - silane film was control group (group A), antibacterial peptide KSL 0.25 mg / ml group B, KSL 0.50 mg / ml group C, KSL 0.75 mg / Group) for the experimental group. Scanning electron microscope (SEM) was used to observe the morphological features of the specimens. CCK-8 was used to detect the cell adhesion and proliferation at different time points. ALP was used to detect the alkaline phosphatase activity at different time points and the number of viable cells on the surface was observed with laser confocal microscopy. Results The results of CCK-8 and ALP showed that the order of proliferation, adhesion and alkaline phosphatase activity of the four groups were D group> C group> B group> A group. The differences among the four groups were statistically significant. The laser Confocal microscopy showed that the higher the antibacterial peptide KSL concentration at the effective bactericidal concentration, the better the antibacterial effect. Conclusion Antibacterial peptide KSL coating has good biocompatibility and antibacterial activity.