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目的研究木豆叶提取物对大鼠心肌缺血再灌注损伤的保护作用。方法采用结扎冠状动脉前降支方法制备大鼠心肌缺血再灌注损伤模型,缺血前10 min大鼠ig给予不同剂量木豆叶提取物(125、200、500 mg/kg),复方丹参滴丸为阳性对照,再灌注30 min后以II导联心电图监测各组大鼠心律失常出现和持续时间,进行心律失常评分;再灌注90 min后测定心肌梗死范围,HE染色观察心肌形态学变化。结果模型组大鼠心律失常出现较早,持续时间较长,室性早搏、室速、室颤等发生率较高,且心律失常在缺血再灌注损伤末期仍非常明显。与模型组相比,木豆叶提取物能够降低大鼠缺血性心律失常的严重程度和发生率,明显缩小心肌梗死范围,减轻心肌细胞肿胀和炎性细胞浸润。结论木豆叶提取物对心肌缺血再灌注损伤大鼠有一定的保护作用。
Objective To study the protective effect of extract of Magnolia biondii on myocardial ischemia-reperfusion injury in rats. Methods The model of myocardial ischemia-reperfusion injury was established by ligation of the anterior descending coronary artery in rats. Different doses of extract (125, 200, 500 mg / kg) Pills were positive control. After 30 min of reperfusion, the occurrence and duration of arrhythmia in each group were monitored by II-lead electrocardiogram. The arrhythmia score was measured. The myocardial infarction range was measured 90 min after reperfusion. The morphological changes of myocardium were observed by HE staining. Results The arrhythmia occurred earlier, longer in duration, premature ventricular contractions, ventricular tachycardia and ventricular fibrillation were higher in model group, and arrhythmia was still evident at the end of ischemia-reperfusion injury. Compared with the model group, the extract of Magnolia biondii can reduce the severity and incidence of ischemic arrhythmia in rats, significantly reduce the scope of myocardial infarction, reduce myocardial cell swelling and inflammatory cell infiltration. Conclusion The extract of Magnolia biondii has a protective effect on myocardial ischemia-reperfusion injury in rats.