肝细胞体外培养技术为肝病及相关学科的研究提供了重要的实验基础 ,促进了肝脏疾病的研究进展。近年 ,肝炎病毒的感染机制、生物人工肝支持系统等临床基础和应用研究已初见成效 ,这一可喜成果与肝细胞体外培养技术的研究进展密切相关 ,本文就近年相关文献综述如下。1　肝细胞的 In vitro culture of hepatocytes provides an important experimental basis for the study of liver diseases and related disciplines, and advances the research progress of liver diseases. In recent years, the clinical basis and application of hepatitis virus infection mechanism, bioartificial liver support system and other clinical studies have begun to bear fruit, and this gratifying achievement is closely related to the progress of hepatocyte culture in vitro research. 1 hepatocyte