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目的分析人群中IL-10-819 C/C和TNF-α-1031 C/C基因型与胃十二指肠疾病的关系,确定携带以上该基因型的的人群罹患胃十二指肠疾病易感性的风险性。为临床诊断和预防这些疾病提供新的思路和方法。方法选取H.pylori阳性的48例慢性胃炎患者,46例十二指肠溃疡患者,51例胃溃疡患者,43例胃癌患者和100例健康对照者,2种基因型分别采用普通PCR和多重引物特异PCR法检测。结果在胃炎组中TNF-A-1031各基因型的频率(T/T,50%;T/C,40%;C/C,10%)与对照组(T/T,73%;T/C,25%;C/C,2%)比较,分布差异有统计学意义(χ2=9.975,P<0.05)。在胃溃疡组中TNF-A-1031各基因型的频率(T/T,49%;T/C,43%;C/C,8%)与对照组(T/T,73%;T/C,25%;C/C,2%)比较,分布差异有统计学意义(χ2=9.464,P<0.001)。在十二指肠溃疡组中TNF-A-1031各基因型的频率(T/T,72%;T/C,26%;C/C,2%)与对照组(T/T,73%;T/C,25%;C/C,2%)比较,分布差异有统计学意义(χ2=9.840,P<0.05)。在胃癌组中TNF-A-1031各基因型的频率(T/T,50%;T/C,41%;C/C,9%)与对照组(T/T,73%;T/C,25%;C/C,2%)比较,分布差异有统计学意义(χ2=9.335,P<0.001);Logistic回归分析与携带TNF-A-1031 T/T者比较,携带TNF-A-1031 C/C者发生胃炎的危险性为OR=7.60(95%CI:1.38-41.77);与携带TNF-A-1031 T/T者比较,携带TNF-A-1031 C/C者发生胃溃疡的危险性为OR=5.84(95%CI:1.00-33.84);与携带TNF-A-1031 T/T者比较,携带TNF-A-1031 C/C者发生十二指肠溃疡的危险性为OR=7.94(95%CI:1.44-43.67);与携带TNF-A-1031 T/T者比较,携带TNF-A-1031 C/C者发生胃癌的危险性为OR=6.95(95%CI:1.19-40.63)。在疾病组和对照组中IL-10-819的各基因型频率的分布差异无统计学意义(P>0.05)。结论 TNF-α-1031基因多态性与胃炎、胃溃疡、十二指肠、胃癌的易感性相关。
Objective To analyze the relationship between the IL-10-819 C / C and TNF-α-1031 C / C genotypes and gastroduodenal diseases in the population and to determine the prevalence of gastroduodenal diseases among those with the above genotype Sensitive risk. For clinical diagnosis and prevention of these diseases provide new ideas and methods. Methods Forty-eight patients with chronic gastritis, 46 patients with duodenal ulcer, 51 patients with gastric ulcer, 43 patients with gastric cancer and 100 healthy controls were enrolled in this study. The two genotypes were selected by ordinary PCR and multiple primers Specific PCR method. Results The frequencies of T / T, T / T, 50%; T / C, 40%; C / C and 10% C, 25%; C / C, 2%), the difference was statistically significant (χ2 = 9.975, P <0.05). The frequencies of T / T, 49%; T / C, 43%; C / C, 8% of TNF-A-1031 genotypes in gastric ulcer group were significantly higher than those in control group C, 25%; C / C, 2%), the difference was statistically significant (χ2 = 9.464, P <0.001). In the duodenal ulcer group, the frequencies of TNF-A-1031 genotypes (T / T, 72%; T / C, 26%; C / ; T / C, 25%; C / C, 2%), the difference was statistically significant (χ2 = 9.840, P <0.05). In the gastric cancer group, the frequencies of T-T, T / C, T / C, C / C and 9% (P <0.001). Logistic regression analysis showed that compared with those carrying TNF-A-1031 T / T, the distribution of TNF-a-1031 T / The risk of developing gastritis at 1031 C / C was OR = 7.60 (95% CI: 1.38-41.77). Gastric ulceration occurred in patients carrying TNF-A-1031 C / C compared with those carrying TNF- (95% CI: 1.00-33.84). The risk of duodenal ulcer with TNF-A-1031 C / C compared with those carrying TNF-A-1031 T / T was OR = 7.94 (95% CI: 1.44-43.67). The risk of gastric cancer with TNF-A-1031 C / C was OR = 6.95 compared with those with TNF- 1.19-40.63). The distribution of IL-10-819 genotype frequency in disease group and control group had no statistical significance (P> 0.05). Conclusion TNF-α-1031 gene polymorphism is associated with susceptibility to gastritis, gastric ulcer, duodenum and gastric cancer.