半薄切片的常规染色方法是用1％甲苯胺蓝在60℃下滴染切片。这种方法虽然简单快速,但技术难度大,常有着色不均,效果不够稳定和难于掌握等弊病,妨碍了半薄切片技术的实际应用。为避免这些缺点,我们进行了本研究。方法与结果一、制作半薄切片:用4％戊二醛和1％四氧化锇双固定家兔心肌组织,Epon812包埋。包埋块在修块机上切厚2μm的半薄切片。二、制备甲苯胺蓝染液:用甲苯胺蓝1.0克、硼砂1.0克和双蒸水100毫升配出1％甲苯胺蓝溶 Conventional staining of semi-thin sections is by dripping the sections with 1% toluidine blue at 60 ° C. Although this method is simple and fast, it is technically difficult, often has uneven coloring, the effect is not stable enough and difficult to grasp other maladies, hinder the practical application of semi-thin slice technology. To avoid these shortcomings, we conducted this study. Methods and results First, the production of semi-thin sections: 4% glutaraldehyde and 1% osmium tetroxide double fixed rabbit myocardial tissue, Epon812 embedded. Embedded block Cut a 2μm thick semi-thin section on a block machine. Second, the preparation of toluidine blue dye: Toluidine blue 1.0 grams, 1.0 grams of borax and double distilled water 100 ml with 1% toluidine blue solution