单克隆抗体在体内和体外诊断及治疗方面的广泛应用,已引起了人们的极大兴趣。许多纯化单克隆抗体的层析技术已经提出,并已成功地应用起来。例如阴离子交换剂、A蛋白、羟磷灰石、疏水性相互作用、DEAE亲和胶兰以及阳离子交换剂等。然而,其中没有那一种方法是万能的。因为不同的单抗隆抗体具有不同的等电点、疏水性、分子量以及生物活性。此外,单克隆抗体所存在的介质也有很大的影响。因此,必须针对欲纯化的单克隆抗体以及每次纯化的材料数量来选择最适方法。高效液相层析(High Performance Liquid The widespread use of monoclonal antibodies in in vivo and in vitro diagnosis and treatment has drawn great interest. Many chromatographic techniques for the purification of monoclonal antibodies have been proposed and have been successfully applied. Such as anion exchangers, protein A, hydroxyapatite, hydrophobic interactions, DEAE affinity gellan and cation exchangers and the like. However, none of these methods is omnipotent. Because different monoclonal antibodies have different isoelectric points, hydrophobicity, molecular weight and biological activity. In addition, the presence of the monoclonal antibody medium also has a great impact. Therefore, the optimal method must be chosen for the amount of monoclonal antibody to be purified and the amount of material to be purified at a time. High Performance Liquid