硫芥的致突变与致癌的作用,早为实验和人类的实践所证实。本文对小鼠(ICR种)硫芥不同剂量中毒诱发骨髓粒细胞微核形成以及在不同剂量条件下,剂量与致突变的相关性进行了研究。硫芥纯度91.1％,按需要配成丙二醇液溶,皮下注射,24h后剪取小鼠后腿骨约1cm,用Hany’s液冲洗髓腔。将骨髓在玻片上推开,自然干燥,常规瑞氏染色,油镜下计数 The mutagenic and carcinogenic effects of sulfur mustard have been confirmed experimentally and in human practice. In this paper, we investigated the relationship between dose and mutagenesis of bone marrow granulocyte micronucleus formation induced by different doses of sulfur mustard in mice (ICR species). The purity of sulfur mustard was 91.1%, and it was dissolved into propylene glycol as required. Subcutaneous injection was performed. After 24 hours, the hind leg bones of the mice were clipped and the marrow cavity was washed with Hany’s solution. The bone marrow was pushed open on the slide, naturally dried, conventional Wright’s staining, and counting under oil mirror