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环介导等温扩增(LAMP)技术是利用2对特殊设计的引物,一种具有链置换活性的DNA聚合酶,在恒温条件下特异、高效地扩增DNA的新技术,扩增产物为一系列反向重复的靶序列构成的茎环结构和多环花椰菜样结构的DNA片段混合物,电泳图谱呈阶梯式带状分布,反应伴有白色的副产物焦磷酸镁沉淀产生,可通过肉眼定性判断反应结果。作为一种新的核酸扩增技术,已被应用于疟疾、血吸虫等寄生虫的研究中。LAMP方法敏感、特异,简便,在寄生虫学研究中有广阔的应用前景。
Loop-mediated isothermal amplification (LAMP) is the use of two pairs of specially designed primers, a DNA polymerase with strand displacement activity, under specific temperature conditions, efficient amplification of DNA, a new technology, amplification products Series of inverted repeat target sequence consisting of stem-loop structure and polycyclic cauliflower-like structure of the DNA fragment mixture, the electrophoresis pattern was banded zonal distribution, the reaction accompanied by white by-product of magnesium pyrophosphate precipitation can be qualitatively judged by the naked eye Reaction result As a new nucleic acid amplification technique, it has been applied to the research of parasites such as malaria and schistosomiasis. The LAMP method is sensitive, specific and simple, and has broad application prospects in the research of parasitology.