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建立了婴幼儿配方乳粉中5种游离核苷酸的准确测定方法.采用等电点沉淀蛋白,强阴离子交换固相萃取柱净化,Atlantis T3色谱柱分离,二极管阵列检测器检测,外标法定量.结果表明:5种核苷酸在0.1～100μg/mL范围内线性关系良好(r≥0.999),平均回收率为95.2％ ～103％,相对标准偏差均小于4.5％.方法可为企业质量控制和政府监管提供有力的技术支撑.“,”A method was developed for the simultaneous determination of five free nucleotides in infant formula by high performance liquid chromatography ( HPLC) . The samples were extracted with water, and the proteins were precipitated by adjusting isoelectric point. The five free nucleotides were purified using a SAX SPE cartridge, separated on an Atlantis T3 column, and detected with diode -array detector by external standard method. The average recoveries for the target compounds were between 95％ ～103％ with RSDs less than 4. 5％. The linearities of five compounds were in the concentration ranges of 0. 1 to 100μg /mL, with the good correlation coefficients (r≥0. 999). The method is easy, accurate and highly reproducible. The developed method can provide strong technical support for enterprise quality control and government regulation.