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目的:探讨贝母素乙对博莱霉素诱导的急性肺损伤大鼠TGF-β/MAPK ERK细胞信号转导通路影响。方法:大鼠麻醉后,气管内注入博莱霉素5mg/kg复制大鼠肺损伤模型,术后第2天开始每天灌胃1ml/100g体重假手术组和模型组,地塞米松0.405mg/kg组,贝母素乙5mg/kg组、贝母素乙2.5mg/kg组。28天后,麻醉并处死大鼠,肺组织固定、包埋、切片,脱蜡后,行Masson三色染色,观察肺间质炎症程度,免疫组化检测肺组织TGF-β1、MEK1/2、ERK1/2、CTGF的水平,观察贝母素乙对肺损伤大鼠TGF-β/MAPK ERK细胞信号转导通路影响。结果:贝母素乙可显著降低大鼠肺间质炎症程度,显著降低肺间质纤维化评分;贝母素乙可显著降低大鼠肺组织TGF-β1、ERK1/2、MEK1/2、CTGF水平,而与地塞米松0.405mg/kg组作用相似,贝母素乙的有效剂量为2.5mg/kg、5mg/kg。结论:贝母素乙可通过显著降低TGF-β/MAPK ERK细胞信号转导水平而减轻博莱霉素诱发的大鼠肺损伤程度。
Objective: To investigate the effect of carbenoxolol on signal transduction pathway of TGF-β / MAPK ERK cells induced by bleomycin-induced acute lung injury in rats. Methods: Rats were anesthetized and bleomycin 5 mg / kg was injected intratracheally to establish a model of lung injury in rats. The rats in the 1 ml / 100 g body weight sham operation group and model group were orally gavaged with dexamethasone 0.405 mg / kg group, 5 mg / kg peiminine group, and 2.5 mg / kg peiminine group. After 28 days, the rats were anesthetized and sacrificed. The lung tissue was fixed, embedded, sectioned and dewaxed. Masson trichrome staining was performed to observe the degree of interstitial lung inflammation. The expression of TGF-β1, MEK1 / 2 and ERK1 / 2, CTGF, and to observe the effects of basifin B on the signal transduction pathway of TGF-β / MAPK ERK in lung injury rats. Results: Fritillaria B could significantly reduce the degree of interstitial inflammation in rats, and significantly reduce the interstitial fibrosis score; Fritillaria B could significantly reduce the expression of TGF-β1, ERK1 / 2, MEK1 / 2, CTGF Level, and 0.405mg / kg dexamethasone group similar effect, the effective dose of peimine B 2.5mg / kg, 5mg / kg. Conclusion: Peiminine B can reduce bleomycin-induced lung injury in rats by significantly decreasing the signal transduction level of TGF-β / MAPK ERK cells.