目的克隆hsa-miR-30e并构建其慢病毒表达载体。方法 PCR扩增hsa-miR-30e的前体序列,克隆于慢病毒载体pll3.7,转染包装细胞293FT细胞,收获病毒颗粒,荧光定量PCR检测hsa-miR-30e的表达。结果经双酶切及测序鉴定,hsa-miR-30e的慢病毒表达载体构建成功,倒置荧光显微镜下观察转染后293FT细胞发出绿色荧光。结论成功构建hsa-miR-30e的慢病毒表达载体,为进一步研究hsa-miR-30e的生物学功能奠定了基础。 Objective To clone hsa-miR-30e and construct its lentivirus expression vector. Methods The precursor sequence of hsa-miR-30e was amplified by PCR, cloned into lentiviral vector pll3.7, transfected into 293FT cells of packaging cells, and the virus particles were harvested. The expression of hsa-miR-30e was detected by quantitative PCR. Results The lentiviral vector of hsa-miR-30e was successfully constructed by double enzyme digestion and sequencing. The green fluorescent of 293FT cells was observed under inverted fluorescence microscope. Conclusion The successful construction of hsa-miR-30e lentiviral vector, which laid the foundation for the further study of the biological function of hsa-miR-30e.