论文部分内容阅读
目的研究干细胞肿瘤标志物CD133、下游信号通路蛋白哺乳动物雷帕霉素靶蛋白(mTOR)与胃癌临床病理特征的关系,分析两者相关性。方法实时荧光定量检测20例胃癌及癌旁正常组织中CD133和mTOR mRNA的表达;免疫组化检测88例胃癌组织CD133和mTOR蛋白的表达,并与胃癌患者的病理资料进行统计分析。结果 CD133及mTOR mRNA水平均显著高于癌旁正常组织,相关性分析显示CD133水平与mTOR mRNA水平呈正相关(r=0.779,P<0.01);免疫组化显示CD133蛋白表达与年龄、性别、肿瘤大小、分化程度及远处转移无显著相关性(P>0.05),但与TNM分期(P=0.007)及淋巴结转移显著相关(P=0.004),mTOR蛋白表达与年龄、性别、肿瘤大小及远处转移无显著相关性(P>0.05),但与分化程度(P=0.041)、TNM分期(P=0.001)及淋巴结转移(P<0.001)显著相关。相关分析显示,CD133与mTOR蛋白表达呈正相关(r=0.226,P=0.034)。结论 CD133与mTOR在胃癌进展及转移中起协调作用,两者高表达,预示肿瘤有高度侵袭及转移潜能。
Objective To investigate the relationship between the stem cell tumor marker CD133, the downstream signaling pathway mammalian target of rapamycin (mTOR) and clinicopathological features of gastric cancer, and to analyze their correlation. Methods The expression of CD133 and mTOR mRNA in 20 cases of gastric cancer and adjacent normal tissues were detected by real-time fluorescence quantitative assay. The expressions of CD133 and mTOR protein in 88 cases of gastric cancer were detected by immunohistochemistry. The pathological data of gastric cancer were statistically analyzed. Results The levels of CD133 and mTOR mRNA were significantly higher than those in adjacent normal tissues. Correlation analysis showed that the level of CD133 was positively correlated with the level of mTOR mRNA (r = 0.779, P <0.01). Immunohistochemistry showed that the expression of CD133 protein correlated with age, sex, (P = 0.007) and lymph node metastasis (P = 0.004). The expression of mTOR protein was significantly correlated with age, sex, tumor size and distant metastasis (P = 0.041), TNM stage (P = 0.001) and lymph node metastasis (P <0.001), but there was no significant correlation between them (P> 0.05). Correlation analysis showed that CD133 and mTOR protein expression was positively correlated (r = 0.226, P = 0.034). Conclusion CD133 and mTOR play a coordinating role in the progression and metastasis of gastric cancer. Both of them are highly expressed, which indicates that the tumor has high invasion and metastatic potential.