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采用Ｎｏｒｔｈｅｒｎ印迹杂交技术分析了大鼠腹腔注射红藻氨酸（ＫＡ）所致急性（注射ＫＡ后一天内）和慢性（注射ＫＡ后１５天）惊厥过程中海马内ｃ—ｆｏｓｍＲＮＡ水平的变化。结果发现：ＫＡ导致急性惊厥过程中海马内ｃ一ｆｏｓｍＲＮＡ水平显著增高，且增高的水平与惊厥行为的严重程度相关；而慢性惊厥过程中海马内ｃ—ｆｏｓｍＲＮＡ水平反而显著下调。这提示ＫＡ所致急性和慢性惊厥过程中ｃ—ｆｏｓ基因处于不同的功能状态。＊ｉｎｄｉｃａｔｅｓＰＭ０．０１ｃｏｍｐａｒｅｄｗｉｔｈｃｏｎｔｒｏｌＮｅｘｔ，ｗｅｅｘａｍｉｎｅｄｔｈｅｅｆｆｅｃｔｏｆｃｈｒｏｎｉｃｓｅｉｚｕｒｅｓｏｎｃ－－ｆｏｓｍＲＮＡｉｎｄｕｃｔｉｏｎｔｏｄｅｔｅｒｍｉｎｅｉｆｌｏｎｇａｌｌｔｅｒｍｒｅｃｕｒｒｅｎｔｓｐｏｎｔａｎｅｏｕｓｓｅｉｚｕｒｅｓａｌｓｏｕｐ－－ｒｅｇｕｌａｔｅｃ－－－ｆｏｓｅｘｐｒｅｓｓｉｏｎ．Ｉｔｗａｓｆｏｕｎｄｕｎｅｘｐｅｃｔｅｄｌｙｔｈａｔｔｈｅｉｎｄｕｃｔｉｏｎｏｆｃ－－ｆｏｓｍＲＮＡｉｎｔｈｅｈｉｐｐｏｃａｍｐｕｓ，ｍｅａｓｕｒｅｄａｔ１５ｄａｙｓａｆｔｅｒＫＡｉｎｊｅｃｔｉｏｎ，ｗａｓｉｎｈｉｂｉｔｅｄｄｕｒｉｎｇｃｈｒｏｎｉｃｓｅｉ
The changes of c-fos mRNA in the hippocampus of rats induced by kenacin (KA) during acute phase (one day after KA injection) and seizures (15 days after KA injection) were analyzed by Northern blotting. The results showed that KA induced a significant increase of c-fos mRNA in hippocampus during acute convulsions, and the increased level correlated with the severity of convulsions. However, c-fos mRNA levels in hippocampus were significantly down-regulated during KA. This suggests that the K-induced acute and chronic seizures during c-fos gene in different functional status. * IndicatesPM0.01comparedwithcontrolNext, weexaminedtheeffectofchronicseizuresonc - fosmRNAinductiontodetermineif longalltermrecurrentspontaneousseizuresalsoup - regulatec --- fosexpression. Itwasfoundunexpectedlythattheinductionofc - fosmRNAinthehippocampus, measuredat15daysafterKAinjection, wasinhibitedduringchronicsei