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目的 :研究碳酸酐酶同功酶Ⅰ、Ⅱ (Carbonicanhydrase、CAⅠ、CAⅡ )在胃癌组织内的表达及意义。方法 :应用免疫组化法测定了 30例早期胃癌 ,30例胃腺瘤型息肉 ,30例轻度不典型增生胃上皮组织中CAⅠ、CAⅡ的表达 ,以 30例慢性浅表性胃炎 (非活动性 )为对照组。结果 :CAⅠ、CAⅡ在早期胃癌组织的阳性率分别为 13 .3 %、2 6 .7% ,胃腺瘤型息肉组织的阳率率分别为 40 %、6 0 % ,轻度不典型增生的胃上皮组织内的阳性率均为 10 0 % ,慢性浅表性胃炎 (非活动性 )的阳性率分别为 93 .3 %、96 .4% ,且早期胃癌组织CAⅠ、Ⅱ活性明显低于胃息肉及不典型增生上皮组织 ,统计学检验差异显著。结论 :CAⅠ、Ⅱ为正常胃粘膜上皮细胞分化、成熟的指标 ,在肿瘤发生早期活性已降低和丧失
Objective: To study the expression and significance of carbonic anhydrase isoenzymes I and II (Carbonicanhydrase, CAI and CAII) in gastric cancer. METHODS: Immunohistochemistry was used to determine the expression of CAI and CAII in 30 cases of early gastric cancer, 30 cases of gastric adenoma polyps, and 30 cases of dysplasia of gastric dysplasia. 30 cases of chronic superficial gastritis (inactive ) for the control group. Results: The positive rates of CAI and CAII in early gastric cancer were 13.3% and 26.7%, respectively. The positive rates of gastric adenoma polyps were 40% and 60%, respectively. The gastric dysplasia was mild. The positive rate in epithelial tissue was 100%, and the positive rate of chronic superficial gastritis (inactive) was 93.3% and 96.4%, respectively. The activity of CAI and II in early gastric cancer was significantly lower than that in gastric polyps. And dysplasia epithelial tissue, statistically significant differences. Conclusion : CAI and II are indexes of differentiation and maturation of normal gastric mucosal epithelial cells. Their activity has been reduced and lost in the early stage of tumorigenesis.