用亚致死量单核细胞增多性利斯特菌感染细胞(2×10~4CFU)给小鼠静脉注射,然后测定血、脾脏和肝脏中的TNF,IL-6和IFN-γ水平.感染24小时时脾脏中TNF开始增多,72小时达高峰.肝脏中TNF滴度呈双峰型,感染1小时升高,4小时达高峰,以后下降到72小时又达高峰.血中未测出TNF;感染1小时时血中测出IL-6,24小时血和脾脏中可测出,48小时 Mice were intravenously injected with sublethally infected Listeria monocytogenes (2 × 10 ~ 4 CFU), and the levels of TNF, IL-6 and IFN-γ in blood, spleen and liver were measured. TNF in the spleen began to increase at 72 hours and peaked at 72 hours.The TNF titers in the liver showed a bimodal pattern with an increase in 1 hour of infection and a peak at 4 hours and reached a peak at 72 hours later.The level of TNF was not detected in the blood, IL-6 was detected in the blood at 1 hour of infection, 24 hours in blood and spleen, and 48 hours