在食用菌的生产过程中,常因杂菌的污染而导致减产或绝收。目前,广大菇农都在寻找一种最理想的消毒剂。笔者对灭菌后的金星消毒液对青霉的杀伤力作了一次试验,现将结果报告如下: (一)供试材料 药剂为湖北随州市化学科研制品厂生产的金星消毒液。菌株是本院特产系微生物实验室自存青霉菌种,培养基为PDA培养基。 (二)试验方法 ①制孢子悬浮液:取青霉孢子置无菌水中,混匀。用血球计数法测得每毫升孢子液含孢子3.05×10~6个。再采用连续稀释法稀释10~3。②试验设计:A处理,在每个平板中加入经1.05kg/cm~230分钟高压灭菌后的30倍金星消毒液0.1ml;B处理,在每个平板中加入0.1ml无菌水作对照。每处理三个重复。每个平板中接入0.1ml上述孢子液,涂布均匀,置25℃恒温箱内培养,观察菌落数及长势。 (三)结果分析 试验结果见下表。从表中可看出:经高压灭菌后的30倍金星消毒液处理的青霉孢子的萌发率,平均比对照降低了5％左右;金星消毒液的药 In the production of edible fungi, often due to contamination of the bacteria led to reduced production or income. At present, mushrooms are looking for a wide range of the best disinfectant. The author of sterilized Venus disinfectant on the lethality of the penicillin made a test, the results are reported as follows: (A) for the test material Pharmacy Hubei Suizhou Chemical Products Factory Venus disinfectant. Strain is a specialty of our hospital microbial strains penicillium self-storage, medium for the PDA medium. (B) test methods ① spores suspension: take the penicillium spores sterile water, mix. Measured with the blood count per ml spore fluid containing spores 3.05 × 10 ~ 6. Then use serial dilution method to dilute 10 ~ 3. ② Experimental design: A treatment, add 0.1ml of 30 times Venus disinfectant after autoclaving 1.05kg / cm ~ 230min to each plate; B treatment, add 0.1ml sterile water to each plate as control . Three replicates per treatment. Access to each plate 0.1ml spore liquid, coating evenly, set 25 ℃ incubator incubator to observe the number of colonies and growth. (C) the results of the test results in the table below. As can be seen from the table, the germination rate of Penicillium spores treated with autoclaved 30 times Venus disinfectant was reduced by about 5% on average compared with the control; Venus disinfectant solution