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目的考察磷酸肌苷钠(sodium inosinmonophosphate,IMP-NA)对过氧化氢(H2O2)诱导的人晶状体上皮细胞SRA01/04(human lens epithelial cells SRA01/04,HLEC SRA01/04)损伤的保护作用,为IMP-NA治疗白内障疾病提供科学依据。方法采用MTT法建立H2O2诱导的HLECSRA01/04氧化损伤模型,根据相应量-效曲线确定H2O2模型的最佳作用浓度及作用时间,同时进行一般形态学观察。考察IMP-NA对HLEC SRA01/04的保护作用。酶联免疫法检测IMP-NA调控凋亡相关基因Bcl-2和Bax的表达情况。结果 H2O2损伤模型最优条件下为最佳作用浓度200μmol.L-1、作用时间24 h,与H2O2处理组比较,浓度为0.1、1.0、10.0、100.0μmol.L-1IMP-NA可显著提高HLEC SRA01/04的存活率(P<0.05)。酶联免疫结果显示,IMP-NA在浓度为1.0～100.0μmol.L-1内显著上调Bcl-2基因的表达、下调Bax基因的表达(P<0.01)。结论 IMP-NA对H2O2诱导的HLEC SRA01/04氧化损伤具有一定的保护作用,其作用机制可能与调控凋亡相关基因Bcl-2和Bax的表达情况有关。
Objective To investigate the protective effect of sodium inosin monophosphate (IMP-NA) on human lens epithelial cells (SRA01 / 04, HLEC SRA01 / 04) induced by hydrogen peroxide (H2O2) IMP-NA treatment of cataract disease provide a scientific basis. Methods H2O2-induced oxidative damage model of HLECSRA01 / 04 was established by MTT method. The optimal concentration and duration of action of H2O2 were determined according to the corresponding dose-response curve. The morphological changes of HLECSRA01 / 04 were also observed. The protective effect of IMP-NA on HLEC SRA01 / 04 was investigated. Enzyme-linked immunosorbent assay was used to detect the expression of apoptosis-related genes Bcl-2 and Bax by IMP-NA. Results Under H2O2 injury model, the best effect concentration was 200μmol.L-1 and the action time was 24 h. Compared with H2O2 treatment group, 0.1,1.0,10.0,100.0μmol.L-1IMP-NA could significantly increase HLEC Survival of SRA01 / 04 (P <0.05). Results of enzyme-linked immunosorbent assay showed that IMP-NA up-regulated the expression of Bcl-2 gene and down-regulated the expression of Bax gene in the concentration of 1.0-100.0 μmol·L-1 (P <0.01). Conclusion IMP-NA can protect H2O2-induced oxidative damage of HLECs induced by SRA01 / 04, and its mechanism may be related to the regulation of the expression of Bcl-2 and Bax.