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已有报道提出老龄化进程可能对伤害性行为表现有一定的影响 ,但是相关的机制仍不很明确。辣椒素受体 VR1(现命名为 TRPV1)已被证实为热伤害性感受器 ,老龄化进程中 TRPV1在痛传导路中的表达水平是否随年龄改变尚不清楚。本研究中应用免疫组织化学方法对三个年龄段 (青龄 :2～ 3月 ;中龄 :17～ 19月 ;老龄 2 4～ 2 6月 )大鼠脊髓背角的 TRPV1的表达进行了观察 ,结果显示 :(1) TRPV1在正常大鼠脊髓背角浅层的分布密度及其分布面积随年龄增长而逐渐减小 ;(2 )在外周致炎状态下 ,脊髓背角 TRPV1免疫反应产物密度在同年龄组内比较 ,青龄组减少 ,中龄和老龄组增加 ,而三年龄组内比较其分布面积时都明显增大 ,并且在非致炎组存在的组间差异消失。在青龄组大鼠 ,TRPV1免疫反应产物密度结果和分布面积结果并不一致 ,其原因未知。综上所述 ,本结果提示正常大鼠脊髓含 TRPV1的纤维终末随年龄增长而减少 ,但是在致炎情况下 ,TRPV1支配面积减少的现象消失。这可能是由于外周炎症刺激重新募集了背根节神经元 ,而老龄大鼠这种能力更强。本实验还提示 ,用免疫组织化学技术检测脊髓化学物质有无变化 ,测量光密度值和测量面积的方法都应该予以考虑
It has been reported that the process of aging may have a certain impact on the performance of harmful behaviors, but the related mechanisms are still not clear. The capsaicin receptor VR1 (now named TRPV1) has been identified as a thermal nociceptor and it is unclear whether the expression level of TRPV1 in pain pathways changes with age during the aging process. In this study, immunohistochemistry was used to detect the expression of TRPV1 in the spinal dorsal horn of three age groups (age: 2-3 months; middle age: 17-19 months; aged 2-4 months) The results showed that: (1) The density and distribution area of TRPV1 in the superficial layer of spinal dorsal horn of normal rats gradually decreased with age; (2) Under peripheral inflammatory conditions, the density of TRPV1 immunoreactive product Compared with the same age group, the young age group decreased, the middle age group and the aging group increased, while the three age groups increased their distribution area significantly, and there was no difference among the non-inflammatory groups. The results of density and distribution area of TRPV1 immunoreactive products in the young age group are not consistent, and the reason for this is unknown. Taken together, the results suggest that TRPV1-terminated fibroepithelial terminals decrease in normal rat spinal cord with increasing age, but diminish the dominance of TRPV1 in the absence of inflammation. This may be due to peripheral inflammatory stimuli to recruit DRG neurons, whereas older rats are more capable. This experiment also suggests that immunohistochemical techniques to detect changes in spinal cord chemical substances, the method of measuring optical density and measurement area should be taken into account