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目的 探讨不同苯浓度作业场所对淋巴细胞微核和 /淋巴细胞百分比高的工人的骨髓CD34+ 细胞数量及单个核细胞AnnexinV ,Fas阳性表达率的影响。方法 对 2 0例淋巴细胞微核和 /淋巴细胞百分比偏高的苯作业工人和 10例正常人 ,采用流式细胞术检测CD34+ 细胞数量 ,单个核细胞AnnexinV和Fas的阳性表达率 ;气相色谱法测定作业场所苯浓度。结果 苯浓度 34～ 5 82mg/m3 作业工人骨髓CD34+ 细胞数量明显低于正常对照组 ,有显著性差异 (P <0 .0 5 ) ,相关分析呈负相关 (P <0 .0 5 ) ,r =- 0 .95。骨髓单个核细胞AnnexinV阳性表达率与Fas阳性表达率明显高于正常对照组 (P <0 .0 5 ) ,相关分析呈正相关 (P <0 .0 5 ) ,r =0 .96 ;苯浓度 8～ 32mg/m3 作业工人骨髓仅CD34+ 细胞数量高于正常对照组。结论 动态测定苯作业工人骨髓CD34+ 细胞数量 ,AnnexinV和Fas阳性表达率对慢性职业性苯中毒有早期诊断和预防意义
Objective To investigate the effect of different concentrations of benzene on the number of bone marrow CD34 + cells and the positive rate of AnnexinV and Fas of the workers with high percentage of micronuclei and lymphocytes in lymphocytes. Methods Twenty cases of benzene workers and 10 normal persons with higher percentage of micronuclei and / or lymphocytes in lymphocytes were detected by flow cytometry. The number of CD34 + cells, the positive rate of AnnexinV and Fas in mononuclear cells were detected by gas chromatography Determination of workplace benzene concentration. Results The number of CD34 + cells in the bone marrow of workers exposed to benzene at a concentration of 34-582 mg / m3 was significantly lower than that of the control group (P <0.05), and the correlation analysis was negatively correlated (P <0.05). The r = - 0 .95. The positive rate of AnnexinV in bone marrow mononuclear cells was significantly higher than that in normal control group (P <0.05), and the correlation analysis showed a positive correlation (P <0.05), r = 0.96; the concentration of benzene 8 The number of CD34 + cells in the bone marrow of ~ 32mg / m3 workers was higher than that of the normal control group. Conclusion The dynamic determination of the number of bone marrow CD34 + cells and the positive rate of AnnexinV and Fas in benzene-exposed workers has an early diagnostic and preventive effect on chronic occupational benzene poisoning