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背景:人类β-防御素主要定位于多种组织的表皮或上皮。在眼表中也存在防御素,但其在眼表中的分布及眼表疾病中的作用尚不十分清楚。目的:观察人类β-防御素在眼表组织中的分布,分析其在眼表疾病中的潜在作用。设计、时间及地点:以眼表组织为观察对象体外对照实验,于2006-10/2007-12在上海交通大学医学院附属新华医院中心实验室和中国科学院上海分院细胞生化所完成。材料:18个炎性结膜样本中包括6个胬肉表面球结膜、4个球结膜囊肿、4个酸烧伤结膜组织、2个热烧伤结膜组织和2个结膜肉芽肿组织;15个炎性角膜样本中包括6个病毒性角膜炎、4个霉菌性角膜炎、3个细菌性角膜炎和2个角膜穿孔伤后摘除眼球;9个尸体眼正常球结膜样本,8个尸体眼正常角膜样本。方法:RT-PCR法及免疫组织化学法检测50份样本人类β-防御素表达。主要观察指标:人类β-防御素1～3三种蛋白因子在正常及炎症眼表组织的表达和定位。结果:RT-PCR检测发现,人类β-防御素1,3在所有被检角结膜组织中均显示阳性;人类β-防御素2在大部分炎症眼表组织中显示阳性,在正常眼表组织几乎不表达。免疫组织化学结果显示,大多数炎性眼表组织中均同时表达人类β-防御素1,2,分布于上皮细胞层,以基底层为主,偶见基质细胞的浸润,仅少数无人类β-防御素2表达;正常角膜和球结膜样本中均有人类β-防御素1表达,分布于上皮细胞层,以基底层为主,仅极少数同时存在人类β-防御素2表达。结论:人类β-防御素1,3呈组成式表达于正常及炎性眼表组织上皮细胞表皮与基底上皮细胞表层和基底层,人类β-防御素2则呈诱导式表达于大多数炎性眼表组织上皮细胞表皮与基底上皮细胞表层和基底层,均以基底层居多;3种人类β-防御素在对抗眼表感染和促进眼表损伤修复中可能起重要作用。
Background: Human beta-defensins are predominantly located on the epidermis or epithelium of various tissues. Defensins are also present in the ocular surface, but its role in ocular surface distribution and ocular surface disorders is not well understood. OBJECTIVE: To observe the distribution of human β-defensin in ocular surface tissues and to analyze its potential role in ocular surface diseases. DESIGN, TIME AND SETTING: An in vitro control experiment with ocular surface tissue as the observational object was performed at the Central Laboratory of Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine and the Cell Biochemistry Institute of Chinese Academy of Sciences from October 2006 to December 2007. MATERIALS: Eighteen inflammatory conjunctiva samples included six pterygium conjunctiva, four conjunctival cysts, four acid burned conjunctival tissues, two thermal burns conjunctival tissues and two conjunctival granuloma tissues. Fifteen inflammatory corneas The sample included 6 viral keratitis, 4 mycotic keratitis, 3 bacterial keratitis and 2 corneal perforation after removal of the eyeball; 9 cadaver eyes with normal spherical conjunctiva samples and 8 cornea normal corneal samples. Methods: RT-PCR and immunohistochemistry were used to detect the expression of human β-defensin in 50 samples. MAIN OUTCOME MEASURES: Expression and localization of human β-defensin 1 to 3 protein factors in ocular surface tissues of normal and inflammatory conditions. Results: The results of RT-PCR showed that human β-defensin 1 and 3 showed positive staining in all examined corneal conjunctiva tissues. Human β-defensin 2 showed positive staining in most inflammatory ocular tissues. In normal ocular surface tissues Almost no expression. Immunohistochemistry results showed that most of the inflammatory ocular surface tissues both expressed human β-defensin 1 and 2, distributed in the epithelial cell layer, basal layer-based, occasional infiltration of stromal cells, only a few human β - Defensin 2 expression; normal cornea and conjunctival samples were all human β-defensin 1 expression, distributed in the epithelial cell layer, mainly basal layer, only a very few simultaneous expression of human β-defensin 2. CONCLUSION: Human β-defensin 1 and 3 are constitutively expressed on the surface and basal layers of the epithelium and epithelial cells of normal and inflammatory ocular epithelial cells. Human β-defensin 2 is inducible in most inflammatory Epidermal epithelial cells in the epidermal epithelium and the basal epithelial cells and the basal layer are mostly basal layer; three human β-defensins may play an important role in the prevention of ocular surface infection and the repair of ocular surface injury.