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本文研究的目的是为了改进百日咳的细菌学诊断。为了培养所检查的材料,采用了牛奶血液琼脂培养基并与鲍两得氏Борде培养基相比较,结果证明本培养基有很大的优点。它能保证百日咳杆菌迅旺的发育并发展为大的菌落。该培养基的配制方法归纳如下:先向溶化的含有1％食盐的5％琼脂中,混入等量的脱脂加热牛奶,然后放入高压灭菌器中在0.5大气压力下煮沸40分钟(在这个时期牛奶发生凝固),将热的混合液通过带有棉花的纱布层加以滤过,并均匀地分注入烧瓶中,然后再放到高压灭菌器中在0.5大气压力下消毒20分钟,向冷却到45°—50°的牛奶琼脂内加入20％脱纤维羊血。为了防止外界杂菌的侵入,可向培养基中加入青霉素。用在百日咳杆菌的纯培养和混有葡萄状球菌的培养物中滴定过的剂量最为适合。以5个单位青霉素加入平皿培养基(20立方厘米)为最适宜和最有效。使用青霉素培养基可以进行棉球的‘深入’取材。用同一白金耳接种于三个平皿内,放在调温器中观察72小时。根据所观察的菌落的典型形态,涂片境检,和在玻璃片上与第一相特异家兔的血清凝集,以及根据家兔皮肤坏死确定为百日咳杆菌。所有的分离培养物,都是革兰氏阴性单形态典型的小杆菌,他们能引起出血现象和很好的被特异血清所凝集。
The purpose of this study is to improve the bacteriology diagnosis of whooping cough. In order to cultivate the materials examined, milk blood agar medium was used and compared with Erdostenbosch Борде medium, which proved to be of great advantage. It ensures the rapid development of Bordetella pertussis and the development of large colonies. The preparation of the medium is summarized as follows: To 5% agar containing 1% salt dissolved first, an equal amount of degreased heated milk is mixed and then placed in an autoclave and boiled at 0.5 atm for 40 minutes Period of milk coagulation), the hot mixture through the gauze with cotton to be filtered, and evenly divided into the flask, and then placed in an autoclave disinfected at 0.5 atm pressure for 20 minutes, cooled To 45 ° -50 ° milk agar added 20% defibrinated sheep blood. In order to prevent the invasion of outside bacteria, penicillin can be added to the medium. The best titration doses used in both pure cultures of B. pertussis and cultures mixed with Staphylococcus aureus are most suitable. 5 units penicillin plate medium (20 cm3) is the most appropriate and most effective. The use of penicillin medium for cotton ’in-depth’ material. Inoculate the same Platinum ear in three petri dishes and keep in a thermostat for 72 hours. Based on the typical morphology of the colonies observed, smear biopsies, and agglutination with sera from the first phase-specific rabbits on glass slides, and Bordetella pertussis from rabbit skin necrosis. All of the isolated cultures, which are typical of Gram-negative single-form bacteria, can cause bleeding and agglutinate well with specific serum.