本文报道了沙门氏菌致突变试验(Ames 试验)检测致癌物和致突变物方法的改进。一、试验菌株Ames 试验使用的是一组需要组氨酸的菌株。每一试验菌株含有不同类型组氨酸操纵子突变(见表1) ,此外,标准试验菌株还有可增强检测致突变物能力的其它突变。新的移码菌株TA 97代替过去标准菌株中不敏感的菌株TA 1537。此菌株有一个外加的胞嘧啶,从而 This article reports an improvement in the methods for the detection of carcinogens and mutagens by the Salmonella mutagenicity test (Ames test). First, the test strain Ames test using a group of histidine-requiring strains. Each test strain contains a different type of histidine operon mutation (see Table 1). In addition, the standard test strain has other mutations that enhance the ability to detect mutagens. The new frameshift strain TA 97 replaces the insensitive strain TA 1537 in the past standard strains. This strain has an extra cytosine, thus