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细胞电泳技术是研究膜结构与功能的手段之一。细胞膜表面电荷密度与辐射损伤、化学修饰、免疫修饰以及肿瘤、白血病、心血管疾患等的发病机理均有一定关系。 本实验,应用细胞电泳技能共检测了15例乳腺癌、13例子宫颈癌患者于放疗前后和60例无癌症健康妇女的外周血红细胞电泳率(RBC—EPM)。癌患均以~(60)Co.γ放射源进行外照射放疗,照射率为56.6R/min,疗程开始的初次照射量:乳腺癌为300R,子宫颈癌为400R;疗程末次照射量均为500R。在初、末两次照射之前及照射后1小时,各取耳血10μl,以2.5mlPH7.4磷酸缓冲液制成红细胞悬液。在25±0.5℃下,用细胞电泳装置观测了细胞电泳率(μ/sec/v/cm)。
Cell electrophoresis is one of the means to study the structure and function of membranes. The surface charge density of cell membranes is related to the pathogenesis of radiation damage, chemical modification, immunomodification, and tumor, leukemia, and cardiovascular disease. In this experiment, the electrophoresis technique was used to detect the peripheral blood red cell electrophoresis rate (RBC-EPM) of 15 breast cancer patients, 13 cases of cervical cancer patients before and after radiotherapy and 60 healthy women without cancer. The cancer patients were treated with external radiation therapy with ~(60)Co.gamma radiation source. The radiation rate was 56.6 R/min. The initial radiation dose at the beginning of the treatment period was 300R for breast cancer and 400R for cervical cancer; the last dose of treatment was both 500R. Before and after the first two irradiations and one hour after the irradiation, 10 μl of ear blood was collected and made into a red cell suspension with 2.5 ml of a pH7.4 phosphate buffer. The cell electrophoresis rate (μ/sec/v/cm) was observed with a cell electrophoresis apparatus at 25±0.5°C.