【摘 要】
:
The infusion of coronavirus disease 2019 (COVID-19) patients with mesenchymal stem cells (MSCs) potentially improves clinical symptoms,but the underlying mechanism remains unclear.We conducted a randomized,single-blind,placebo-controlled (29 patients/grou
【机 构】
:
Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences,School of Basic Medicine,Pek
论文部分内容阅读
The infusion of coronavirus disease 2019 (COVID-19) patients with mesenchymal stem cells (MSCs) potentially improves clinical symptoms,but the underlying mechanism remains unclear.We conducted a randomized,single-blind,placebo-controlled (29 patients/group) phase Ⅱ clinical trial to validate previous findings and explore the potential mechanisms.Patients treated with umbilical cord-derived MSCs exhibited a shorter hospital stay (P =0.0198) and less time required for symptoms remission (P =0.0194) than those who received placebo.Based on chest images,both severe and critical patients treated with MSCs showed improvement by day 7 (P =0.0099) and day 21 (P =0.0084).MSC-treated patients had fewer adverse events.MSC infusion reduced the levels of C-reactive protein,proinflammatory cytokines,and neutrophil extracellular traps (NETs) and promoted the maintenance of SARS-CoV-2-specific antibodies.To explore how MSCs modulate the immune system,we employed single-cell RNA sequencing analysis on peripheral blood.Our analysis identified a novel subpopulation of VNN2+ hematopoietic stem/progenitor-like (HSPC-like) cells expressing CSF3R and PTPRE that were mobilized following MSC infusion.Genes encoding chemotaxis factors — CX3CR1 and L-selectin — were upregulated in various immune cells.MSC treatment also regulated B cell subsets and increased the expression of costimulatory CD28 in T cells in vivo and in vitro.In addition,an in vivo mouse study confirmed that MSCs suppressed NET release and reduced venous thrombosis by upregulating kindlin-3 signaling.Together,our results underscore the role of MSCs in improving COVID-19 patient outcomes via maintenance of immune homeostasis.
其他文献
WRKY转录因子家族在植物应对非生物和生物胁迫的防卫反应中起重要作用.岷江百合(Lilium regale Wilson)是高抗枯萎病的野生百合,该研究基于前期转录组测序分析,采用RT-PCR方法从岷江百合中克隆得到WRKY转录因子基因LrWRKY4并分析其功能,以探讨岷江百合对枯萎病菌尖孢镰刀菌(Fusarium oxysporum)侵染的转录调控机制,为进一步研究岷江百合WRKY基因家族的功能奠定基础.结果显示:(1)LrWRKY4开放阅读框为993 bp,编码330个氨基酸,LrWRKY4含有一个高
以成都市为例,构建了全面且系统的社会人文指标体系,包含认识途径、考虑因素和结婚目的3个维度共27个指标,把研究对象分为青年女性、青年男性、中年女性和中年男性4个群体,对不同年龄段的居民婚恋选择的影响因素进行分析.结果表明,随着年龄的增加,男女对于对方的发展潜力和身高长相的要求逐渐降低,对生活习惯和支持陪伴的需求在升高.“门当户对”这一传统的观点仍然影响当代城市居民的婚恋选择,认识途径对于婚恋选择的影响不明显.女性比同年龄段的男性在婚恋选择中更加挑剔,考虑的因素也更多.随着年龄增长,选择空间逐渐减小,对于配
以藏传佛教圣地塔尔寺为例,采用探索性因子分析和K-均值聚类分析法探究来访游客的旅游动机,并依据动机的差异来划分游客类别.结果表明,来访塔尔寺游客的出游动机主要呈现4个维度:精神感召、观光游览、学习探索、情感联系.观光游览是首要的动机,其次是情感联系和精神感召,学习探索是最弱的动机,旅游者出游动机的多样性反映出塔尔寺景区满足了不同游客的旅游诉求.依据旅游动机,该地游客又可分为3类:社交探索型、休闲游览型、朝圣求佛型,其中休闲游览型游客占比最多,朝圣求佛型游客居其次,社交探索型游客占比最少,这是宗教圣地在旅游
The emergence of severe acute respiratory syndrome corona-virus 2 (SARS-CoV-2) variants and new coronaviruses raise the demand for universal vaccines.In recent Science and Cell papers,Martinez et al.and Nathan et al.reported two novel approaches for vacci
The refractory response of pancreatic ductal adenocarcinoma(PDAC) to multiple treatment regimens can be attributed to the presence of a desmoplastic stroma together with an immunosuppressive tumor microenvironment.In a recent study published in Cell,Koika
以乌鲁木齐市为例,分析了多民族聚居城市基础教育设施空间布局特征,并采用社会区分析和多因子相关性检验,研究教育设施布局的社会空间差异.结果表明,乌鲁木齐市教育设施布局呈现核心-边缘结构特征,多数学校集中在天山区和沙依巴克区.扇面分析发现,教育设施以北偏西方向布局为主,教育设施布局沿城市发展主方向拓展.教育设施可达性和数量总体上与城市人口密度相协调,但存在一定空间错配.汉族和维吾尔族聚居街区可达性较高,哈萨克族和回族聚居街区可达性较差,就学人口与可达距离和学校数量负相关.按社区类型分类,工业型街区公办学校数量
Nucleic acid-based antiviral systems,including CRISPR/Cas and RNA interference,play important roles in antiviral defense.In a paper recently published in Cell Research,Wu et al.revealed a novel nucleic acid-based antiviral mechanism against RNA viruses in
True-seed potato has been the holy grail of potato breeding for decades;genetic improvement,notably resistance breeding,has lagged behind in this vegetatively propagated,polyploid staple crop.A recent study by Zhang et al.in Cell shows how genomics can gr
Sphingosine-1-phosphate (S1P) is an important bioactive lipid molecule in cell membrane metabolism and binds to G protein-coupled S1P receptors (S1PRs) to regulate embryonic development,physiological homeostasis,and pathogenic processes in various organs.
Coronavirus disease 2019 (COVID-19),caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2),is the ongoing global pandemic that poses substantial challenges to public health worldwide.A subset of COVID-19 patients experience systemic inflam