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目的观察大黄素对重症急性胰腺炎(SAP)大鼠肠系膜血管周围肥大细胞脱颗粒的干预作用。方法选用雄性Wistar大鼠,随机分为正常对照组、大黄素对照组、DMSO对照组、模型组、大黄素提前干预组、大黄素造模后干预组,每组8只;逆胰胆管注入3%牛磺胆酸钠诱导SAP模型,造模后24 h处死各组大鼠;HE染色观察胰腺组织病理学变化,从水肿、腺泡细胞坏死、炎症及出血等方面对胰腺损伤进行病理学评分;生化方法测血清淀粉酶水平;甲苯胺蓝染色肥大细胞,计算肥大细胞脱颗粒率。结果大黄素提前干预和造模后干预均能减轻SAP大鼠胰腺病理变化,胰腺水肿、腺泡细胞坏死、炎症及出血评分均显著低于模型组;与模型组比较,大黄素提前干预和造模后干预血清淀粉酶水平降低,但差异无统计学意义;甲苯胺蓝染色显示模型组肠系膜血管周围出现密集的肥大细胞,部分肥大细胞呈脱颗粒状态;与模型组肥大细胞脱颗粒率(0.31±0.10)比较,大黄素提前组(0.22±0.10)和造模后干预组(0.23±0.05)均显著降低。结论大黄素能减轻SAP大鼠胰腺病理改变,减少肠系膜血管周围肥大细胞的脱颗粒率。
Objective To observe the intervention of emodin on degranulation of mesenteric perivascular mast cells in rats with severe acute pancreatitis (SAP). Methods Male Wistar rats were randomly divided into normal control group, emodin control group, DMSO control group, model group, emodin intervention group, emodin intervention group, each group of 8; % Sodium taurocholate induced SAP model rats were sacrificed 24 h after the model rats were sacrificed; the pathological changes of the pancreas were observed by HE staining and the pathological score of pancreatic injury was evaluated from the aspects of edema, acinar cell necrosis, inflammation and hemorrhage ; Biochemical methods to measure serum amylase levels; toluidine blue staining mast cells, mast cell degranulation rate was calculated. Results Emodin pretreatment and post-intervention could reduce pathological changes of pancreas, pancreatic edema, acinar cell necrosis, inflammation and hemorrhage score in SAP group were significantly lower than model group; compared with model group, emodin intervention in advance The level of serum amylase in the model group was lower but the difference was not statistically significant. Toluidine blue staining showed dense mast cells around the mesenteric vessels in the model group and some mast cells were degranulation. Compared with the model group, the degranulation rate of mast cells (0.31 ± 0.10), both emodin (0.22 ± 0.10) and post-intervention group (0.23 ± 0.05) were significantly decreased. Conclusion Emodin can relieve the pathological changes of pancreas in SAP rats and reduce the degranulation rate of mesenteric blood vessels around the mast cells.