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目的探讨Visfatin在油酸诱导的SW872成熟脂肪细胞胰岛素抵抗(IR)中的作用。方法体外培养SW872前脂肪细胞,当细胞完全汇合后,加入0.6mmol·L-1油酸诱导分化48h,此时SW872前脂肪细胞被诱导分化为成熟脂肪细胞,以SW872成熟脂肪细胞为研究对象,采用1.0mmol·L-1油酸诱导SW872成熟脂肪细胞产生IR。将其分为正常对照组(成熟SW872脂肪细胞)和IR组(采用1.0mmol·L-1油酸诱导SW872成熟脂肪细胞产生IR)。采用2-脱氧-[3H]-右旋葡萄糖掺入法检测2组SW872成熟脂肪细胞基础状态(基础状态组)、Visfatin和Insulin刺激状态(Visfatin和Insulin刺激组)下的葡萄糖转运能力。结果1.在正常对照组中,Visfatin和Insulin刺激组SW872成熟脂肪细胞葡萄糖转运率显著增加,分别是基础状态组的1.10倍(P<0.01)和1.34倍(P<0.01)。2.在IR组中,SW872成熟脂肪细胞的葡萄糖转运率均显著下降。与正常对照组比较,Visfatin刺激组和基础状态组的葡萄糖转运率分别减少12.30%(P<0.01)和9.73%(P<0.05);Insulin刺激组和基础状态组的葡萄糖转运率分别减少26.81%(P<0.01)和10.50%(P<0.05)。3.IR组内比较,Insulin刺激组与基础状态组比较葡萄糖转运率差异无统计学意义(P>0.05);Visfatin刺激组较基础状态组的葡萄糖转运率增加了6.9%(P<0.05)。结论Visfatin可促进SW872成熟脂肪细胞的葡萄糖转运,增加胰岛素敏感性,改善IR。
Objective To investigate the role of Visfatin in oleic acid-induced insulin resistance (IR) in mature adipocytes of SW872. Methods SW872 preadipocytes were cultured in vitro. When the cells were completely confluent, 0.6 mmol·L-1 oleic acid was added to induce differentiation for 48 h. At this time, SW872 preadipocytes were induced to differentiate into mature adipocytes and SW872 mature adipocytes were studied. 1.0 mmol·L-1 oleic acid was used to induce SW872 mature adipocytes to produce IR. The cells were divided into normal control group (mature SW872 adipocytes) and IR group (IR was induced by inducing SW872 mature adipocytes with 1.0 mmol·L-1 oleic acid). The glucose transport ability of SW872 mature adipocytes in basal state (basal state), Visfatin and Insulin stimulated state (visfatin and Insulin stimulated group) was measured by 2-deoxy- [3H] -d-glucose incorporation assay. In normal control group, glucose transport rate of SW872 mature adipocytes was significantly increased in Visfatin and Insulin-stimulated groups, which were 1.10 times (P <0.01) and 1.34 times (P <0.01) higher than those in basal state group respectively. 2. In IR group, the glucose transport rate of SW872 mature adipocytes decreased significantly. Compared with the normal control group, the glucose transport rate of Visfatin stimulation group and basal state group were decreased by 12.30% (P <0.01) and 9.73% (P <0.05), respectively. Glucose transport rate of Insulin stimulation group and basal state group were reduced by 26.81% (P <0.01) and 10.50% (P <0.05) respectively. There was no significant difference in glucose transport rate between Insulin stimulation group and basal state group in IR group (P> 0.05). Glucose transport rate of Visfatin stimulation group increased by 6.9% (P <0.05) compared with basal state group. Conclusion Visfatin can promote glucose transport in SW872 mature adipocytes, increase insulin sensitivity and improve IR.