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本文报道了对一个与水稻香味基因紧密连锁的ＤＮＡ标记的鉴定。该标记是通过以１２６个已作好连锁图的水稻染色体组克隆、ｃＤＮＡ克隆和燕麦ｃＤＮＡ克隆为杂交探针与含有来自一对有和没有香味基因的近等基因系的ＤＮＡ的Ｓｏｕｔｈｅｒｎ印迹作ＤＮＡ杂交而鉴定出来的。从香味基因供体的染色体组中渗入的染色体片段在近等基因系对之间可以识别出来。带有香味基因的克隆的连锁关系通过用有香味分离的Ｆ３代群体进行证实。香味表现型和来自供体的香味基因具有一致性分离指出该ＤＮＡ标记和该基因间存在有连锁关系。ＲＦＬＰ分析表明，该基因与一个单拷贝ＤＮＡ克隆ＲＧ２８在第８染色体上连锁，图距为４．５ｃＭ。在水稻育种中，对与香味基因连锁的ＤＮＡ标记的鉴定，可能有助于对香味的早代选择。
This paper reports the identification of a DNA marker that is closely linked to rice scented genes. The marker was obtained by Southern blotting of 126 cloned rice genomic clones, cDNA clones and oat cDNA clones as hybridization probes with DNA containing a pair of near-isogenic lines with and without aroma genes Hybridization and identified. The chromosomal segments that have penetrated from the genome of the scent gene donor are identifiable between near-isogenic pairs. The linkage of clones with the aroma gene was confirmed by using a population of F3 generation separated by aroma. The consistent separation of the scent phenotype and the scent gene from the donor indicates that there is a linkage between the DNA marker and the gene. RFLP analysis showed that this gene was linked to RG28, a single copy DNA clone, on chromosome 8 with a map distance of 4.5 cM. In rice breeding, the identification of DNA markers linked to scent genes may contribute to the early selection of scent.