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目的用大鼠外周血双核淋巴细胞微核测试法（ＣＢＭＮＴ），在哺乳动物整体水平，研究猕猴桃汁抗环磷酰胺（ＣＰ）的致突变作用以及生物转化Ⅱ相酶的作用。方法测定大鼠外周血双核淋巴细胞微核细胞率及肝组织中总谷胱甘肽硫转移酶（ＧＳＴ）、尿苷二磷酸葡萄糖醛酸转移酶（ＵＤＰＧＴ）、谷氨酰胺转肽酶（γＧＴ）活性。结果猕猴桃汁对ＣＰ诱发的大鼠外周血双核淋巴细胞微核细胞率有显著抑制作用，能明显诱导大鼠肝脏总ＧＳＴ、ＵＤＧＴＰ活性，但对γＧＴ活性无显著影响。大鼠外周血双核淋巴细胞微核细胞率与总ＧＳＴ、ＵＤＧＴＰ活性呈明显负相关。结论猕猴桃汁抗ＣＰ致微核形成作用的机理可能是通过诱导机体外来化合物代谢解毒酶系，加速ＣＰ的代谢灭活
Objective To investigate the mutagenicity of anti-cyclophosphamide (CP) in kiwi fruit juice and the role of biotransformation phase II enzyme in the whole mammalian level using rat peripheral blood lymphocyte micronucleus test (CBMNT). Methods The rate of micronucleated cells in peripheral blood lymphocytes was measured and total glutathione-S-transferase (GST), uridine diphosphate glucuronosyltransferase (UDPGT), and glutamyl transpeptidase (γ) in liver tissues were measured. GT) activity. RESULTS: Kiwifruit juice significantly inhibited the rate of micronuclear cells in rat peripheral blood lymphocytes induced by CP and significantly induced total GST and UDGTP activity in rat liver, but had no significant effect on γGT activity. The rate of micronucleated cells in peripheral blood lymphocytes was negatively correlated with total GST and UDGTP activity. Conclusion The mechanism of anti-CP micronucleus formation in kiwifruit juice may be induced by induction of metabolism and detoxification enzyme system in vitro to accelerate metabolic inactivation of CP.