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本文应用了附有摄影装置的倒置显微镜与缩时定格显微电影摄影观察了人巨噬细胞体外细胞毒作用。这观察发现了效应细胞与靶细胞之间戏剧性的变化:巨噬细胞细胞毒对肿瘤细胞的杀伤必须通过这两种细胞的膜接触这一步骤。本实验分别观察了胃癌患者、良性胃疾患患者以及正常人的巨噬细胞体外细胞毒作用,发现这些细胞毒作用有强弱程度的不同。实验还发现胃癌患者的巨噬细胞经预培养后对胃癌细胞株仍有细胞毒作用。还看到胃癌患者巨噬细胞培养上清液不影响靶细胞的生长。本文最后对实验观察的结果进行了讨论。
In this paper, the in vitro cytotoxicity of human macrophages was observed using an inverted microscope attached with a photographic device and time-lapse fixed-frame microphotography. This observation revealed a dramatic change between the effector cells and the target cells: macrophage cytotoxicity The killing of tumor cells must go through the membrane contact of these two cells. In this experiment, we observed the cytotoxicity of macrophages in patients with gastric cancer, benign stomach disorders, and normal individuals, and found that these cytotoxic effects were different. The experiment also found that macrophages in gastric cancer patients still had cytotoxic effects on gastric cancer cell lines after preculture. It is also seen that the macrophage culture supernatant of gastric cancer patients does not affect the growth of target cells. At the end of this paper, the results of experimental observations are discussed.