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为吉林省野生黄芩种质鉴定提供参考,为建立黄芩鉴定标准化体系提供依据。使用中药DNA条形码鉴定技术,对目的片段进行扩增并分析,比较不同产地黄芩样本ITS2和psbA-trnH条形码片段的差异,寻找鉴别位点。10个不同产地野生黄芩药材的ITS2和psbA-trnH序列比对结果显示,在232 bp(ITS2)+318 bp(psbA-trnH),共550 bp的序列比对中,仅检测到1个“G”位点的插入/缺失,该位点位于ITS2矩阵的第53 bp处。在吉林省长春、向海、前郭、镇赉、洮南5个地区的野生样品中检测到1个“G”位点的插入/缺失,其他5个省份的样品中无此位点。
It provides a reference for the identification of wild Scutellaria baicalensis in Jilin Province and provides the basis for the establishment of Scutellariae identification standardization system. Using the Chinese traditional medicine DNA barcode identification technology, the target fragment was amplified and analyzed, and the differences of the ITS2 and psbA-trnH barcode fragments in different areas of Scutellaria baicalensis were compared to find the identification sites. ITS2 and psbA-trnH alignments of wild Scutellaria baicalensis Georgi in 10 different areas showed that in the sequence alignment of 232 bp (ITS2) +318 bp (psbA-trnH) with 550 bp in total, only 1 “ G ”insertion / deletion at the 53th bp of the ITS2 matrix. The insertion / deletion of one “G” locus was detected in wild samples from Changchun, Xianghai, Qian Guo, Zhenlai and Taonan in Jilin Province, but not in the other five provinces.