论文部分内容阅读
目的 观察鼠三叉神经节中神经肽P物质、降钙素基因相关肽及神经肽Y阳性神经元的分布与密度,为今后研究神经肽在口腔颌面部的作用提供形态学依据。方法 SD雄性大白鼠8只,灌注固定,取双侧三叉神经节水平位连续切片,ABC法漂染,镜检,计算机图像分析。结果 三叉神经节中有丰富而均匀分布的P物质及降钙素基因相关肽阳性胞体,分别占神经元总数的15%和40%左右,染色深浅不一,主要为中小型假单极细胞,其阳性纤维聚集成束,远心端加入上、下颌神经及眼神经,近心端通向中枢神系统,但三叉神经节中无神经肽Y阳性胞体,只有散在阳性纤维分布。结论 三叉神经节中有丰富的P物质和降钙素基因相关肽神经元,但无神经肽Y神经元。
Objective To observe the distribution and density of neuropeptide substance P, calcitonin gene related peptide and neuropeptide Y positive neurons in the trigeminal ganglion of rats to provide the morphological basis for the future study of the role of neuropeptides in oral and maxillofacial regions. Methods Eight male Sprague Dawley rats were perfused and fixed. Bilateral trigeminal ganglion (Trigeminal ganglionectomy) serial sections were stained with ABC method. Microscopic examination and computer image analysis were performed. Results There were abundant and uniformly distributed substance P and calcitonin gene-related peptide positive cell bodies in the trigeminal ganglion, accounting for about 15% and 40% of the total number of neurons, respectively, with different shades of staining, which were mainly small and medium sized pseudomorphic cells, The positive fibers gathered into bundles, the distal end of the upper and lower jaw nerves and oculomotor nerve, the proximal end leading to the central nervous system, but no neuropeptide Y positive cells in the trigeminal ganglion, scattered only in the distribution of positive fibers. Conclusion The trigeminal ganglion is rich in substance P and calcitonin gene-related peptide neurons but not neuropeptide Y neurons.