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利用壳聚糖与纳米金良好的生物相容性及蛋白固定能力,制备了兼具导电性和透光性的人免疫球蛋白G(IgG)修饰膜,用于修饰玻碳电极,研制了新型电化学发光免疫传感器,并通过扫描电镜(SEM)及交流阻抗技术(EIS)考查了传感器表面性质.基于竞争免疫分析模式,以Ru(bpy)32+标记的羊抗人IgG为发光示踪物,采用新型共反应剂二丁基乙醇胺(DBAE)对光信号进行放大,建立了人IgG的检测方法,线性范围20ngmL-1～1.0μgmL-1,检测限6.5ngmL-1.将该电化学发光传感器应用于人血中IgG的检测,结果令人满意.
Using the good biocompatibility and protein immobilization ability of chitosan and gold nanoparticles, a modified and immunoconductive G (IgG) modified membrane was prepared for the modification of glassy carbon electrode. A novel Electrochemical luminescence immunoassay was used to investigate the surface properties of the sensor by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Based on the competitive immunoassay mode, Ru (bpy) 32+ labeled goat anti-human IgG , A novel co-reactant dibenzylethanolamine (DBAE) was used to amplify the optical signal to establish a method for the detection of human IgG with a linear range of 20 ng mL -1 to 1.0 μg mL -1 and a detection limit of 6.5 ng mL -1. The electrochemical luminescence The sensor is applied to the detection of IgG in human blood and the result is satisfactory.