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目的探讨葡萄籽原花青素(GSP)对PC12细胞氧化损伤的神经保护作用及机制。方法用H2O2作用于PC12细胞,MTT检测细胞活性,用流式细胞仪测定PC12细胞凋亡率,共聚焦显微镜观察细胞Caspase-3活性的变化。结果与模型组比较100μg/ml葡萄籽原花青素可使PC12细胞活性升高(P<0.05)和凋亡率下降(P<0.01),并可抵抗H2O2诱发的PC12细胞Caspase-3活性增强(P<0.01)。结论葡萄籽原花青素可抑制H2O2诱导的PC12细胞凋亡,其机制可能与抑制Caspase-3活化有关。
Objective To investigate the neuroprotective effect of grape seed proanthocyanidins (GSP) on oxidative damage of PC12 cells and its mechanism. Methods PC12 cells were treated with H2O2. The cell viability was measured by MTT assay. The apoptosis rate of PC12 cells was determined by flow cytometry. The changes of Caspase-3 activity were observed by confocal microscopy. Results Compared with the model group, proanthocyanidin of 100μg / ml could increase the activity of PC12 cells and decrease the apoptosis rate of PC12 cells (P <0.01), and increase the activity of Caspase-3 of PC12 cells induced by H2O2 (P < 0.01). Conclusion Grape seed proanthocyanidins can inhibit the apoptosis of PC12 cells induced by H2O2, which may be related to the inhibition of Caspase-3 activation.