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目的探讨胶质瘤组织中血小板源性内皮细胞生长因子(PD-ECGF)、肝细胞生长因子(HGF)的表达及与肿瘤血管形成的关系。方法用逆转录聚合酶链反应(RT-PCR)测定了30例胶质瘤和6例正常脑组织的PD-ECGF mRNA水平,用NIH Image软件量化分析目标PCR条带的面积与吸光度;采用原位杂交法检测HGF mRNA的表达;用免疫组化法检测CD34的表达,计算徽血管密度(MVD),并结合临床和病理资料进行分析。结果胶质瘤组织中PD-ECGF基因呈过度表达,高级别(Ⅲ～Ⅳ级)胶质瘤组织中PD-ECGF mRNA的平均表达水平(0.73±0.18)显著高于低级别(Ⅰ～Ⅱ级)胶质瘤(0.47±0.33)及正常脑组织(0.11±0.17),三组比较差异有显著性(P=0.000);生存时间≥2年组中PD-ECGF mRNA的表达水平(0.47±0.29)低于生存时间<2年组(0.81±0.11),差异有显著性(P=0.001)。HGF mRNA在正常脑组织均未见表达,在低级别和高级别胶质瘤组织中阳性率分别为35.7%和75.0%,差异有显著性(P=0.0301;生存时间≥2年组中HGF mRNA的阳性表达率(27.8%)低于生存时间<2年组(83.3%),差异有显著性(P=0.003)。MVD在正常脑组织、低级别和高级别胶质瘤组织中分别为(8±4)个、(20±7)个和(29±6)个,差异有显著性(P=0.000);MVD和PD-ECGF mRNA、HGF mRNA之间存在显著正相关(r_1=0.390.P_1=0.033;r_2=0.514,P_2=0.004),PD-ECGF mRNA和HGF mRNA之间存在显著正相关(r=0.370,P=0.044)。结论胶质瘤中MVD比正常脑组织显著增高,显示了胶质瘤丰富血供的肿瘤血管生成特征。PD-ECGF、HGF在胶质瘤中的表达显著增强,在肿瘤血管生成中起着重要作用,能共同调节胶质瘤的血管形成,可用于判断胶质瘤的生物学行为,并对判断预后有指导意义。
Objective To investigate the expression of platelet-derived endothelial cell growth factor (PD-ECGF) and hepatocyte growth factor (HGF) in gliomas and its relationship with tumor angiogenesis. Methods The PD-ECGF mRNA levels in 30 gliomas and 6 normal brain tissues were determined by reverse transcription-polymerase chain reaction (RT-PCR). The area and absorbance of the target PCR bands were quantified by NIH Image software. The hybridization method was used to detect the expression of HGF mRNA. The expression of CD34 was detected by immunohistochemistry and the MVD was calculated. The clinical and pathological data were used to analyze the expression of HGF mRNA. Results The expression of PD-ECGF gene in glioma tissues was overexpressed, and the average expression level of PD-ECGF mRNA in high grade gliomas (0.73 ± 0.18) was significantly higher than that in low grade gliomas ) Glioma (0.47 ± 0.33) and normal brain tissue (0.11 ± 0.17), there was significant difference between the three groups (P = 0.000). The expression level of PD-ECGF mRNA in the survival time ≥ 2 years group (0.47 ± 0.29 ) Was lower than the survival time <2 years (0.81 ± 0.11), the difference was significant (P = 0.001). The positive rates of HGF mRNA in normal and low-grade gliomas were 35.7% and 75.0%, respectively, with significant difference (P = 0.0301; HGF mRNA in survival time ≥ 2 years (27.8%) was lower than the survival time <2 years (83.3%) (P = 0.003) .MVD in normal brain tissue, low grade and high grade glioma tissues were ( 8 ± 4), (20 ± 7) and (29 ± 6) respectively. The difference was significant (P = 0.000). There was a significant positive correlation between MVD and PD-ECGF mRNA and HGF mRNA (r_1 = 0.390; P = 0.033; r_2 = 0.514, P_2 = 0.004). There was a significant positive correlation between PD-ECGF mRNA and HGF mRNA (r = 0.370, P = 0.044) .Conclusion MVD in glioma is significantly higher than that in normal brain tissue Glioma rich blood supply of tumor angiogenesis characteristics.PD-ECGF, HGF expression was significantly increased in gliomas, plays an important role in tumor angiogenesis, can jointly regulate glioma angiogenesis, can be used to Judge the biological behavior of glioma, and to guide the prognosis.