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作者报道了以一种反向蛋白A溶血空斑试验,对麻风病人和健康人对照者外周血淋巴细胞分别在用麻风分支杆菌(ML)和EB病毒(EBV)刺激前后Υ-干扰素(Υ-INF)产生细胞数的研究结果.受试的麻风病人BT和LL各10例,均正接受化疗,但无一例用皮质类固醇治疗,其中一些BT病人有逆向反应的症状.对照者分两组,10名在麻风流行区居住不到两周的健康高加索人为一组(未接触ML组),5名在被ML污染的环境中长期工作的健康人为另一组(已接触ML组),方法是从受试者静脉采血,分离淋巴细胞,悬于含青、链霉素及10%人AB型血清的RPMI培养基中(细胞浓度为10~6/ml),置塑料试管内寸37℃、5%CO_2条件下孵育18小时,同一受试者的淋巴细胞孵育时,分别
The authors reported an anti-protein A haemolytic plaque assay of peripheral blood lymphocytes in leprosy patients and healthy controls before and after stimulation with Mycobacterium leprae (ML) and Epstein-Barr virus (EBV) -INF) .There were 10 leptospirosis patients, BT and LL, who were both receiving chemotherapy and none of them were treated with corticosteroids.Among them, some patients with BT had adverse reactions.Controls were divided into two groups , 10 healthy Caucasians living in less than two weeks in the endemic area of leprosy (untreated ML group), five healthy people working long term in an ML-contaminated environment (another group contacted with the ML group), the method Blood samples were taken from venous donors, lymphocytes were isolated, suspended in RPMI medium containing 10% fetal bovine serum, streptomycin, and 10% human AB serum. , 5% CO 2 under the conditions of 18 hours incubation, the same subject’s lymphocyte incubation, respectively