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AIM:To investigate the effects of KAI1/CD82 on biologicalbehavior of colorectal carcinoma cells.METHODS:KAI1 cDNA was transfected into highlymalignant colorectal carcinoma cell line,LoVo,which hadlow level of endogenous KAI1 expression,and establishedstable transfectant clones with high KAI1/CD82 expression.The cell-cell adhesion,cell aggregation,cell-matrix adhesionand cell invasion assay were performed to determine whetherKAI1 transfectant could have an effect on proliferation,adhesion and tumor metastasis in comparison with thecontrol transfectant cells.RESULTS:KAI1 expression did not alter in vitro cellproliferation.But the KAI1 transfectant cells exhibitedsignificantly increased homotypic cell-cell adhesion and cellaggregation in comparison with the control transfectant cells(P<0.05).Furthermore,KAI1 expression significantlysuppressed the cell adhesion to extracellular matrixcomponents and in vitro cell invasion in KAIl-transfectedLoVo cells.The data indicated that KAI1 expressionsignificantly suppressed the metastatic potential of KAI1-transfected LoVo cells.CONCLUSION:Our results suggest that KAI1 mightfunction as a negative regulator of colorectal carcinomametastasis.
AIM: To investigate the effects of KAI1 / CD82 on biological behavior of colorectal carcinoma cells. METHODS: KAI1 cDNA was transfected into highly pureignant colorectal carcinoma cell line, LoVo, which had low level of endogenous KAI1 expression, and established stage transfectant clones with high KAI1 / CD82 expression The cell-cell adhesion, cell aggregation, cell-matrix adhesion and cell invasion assay were performed to determine whether KAI1 transfectant could have an effect on proliferation, adhesion and tumor metastasis in comparison with thecontrol transfectant cells .RESULTS: KAI1 expression did not alter in vitro cellproliferation.But the KAI1 transfectant cells exhibited significantly increased homotypic cell-cell adhesion and cellaggregation in comparison with the control transfectant cells (P <0.05) .Furthermore, KAI1 expression significantlysuppressed the cell adhesion to extracellular matrixcomponents and in vitro cell invasion in KAI1-transfectedLoVo cells The data indicates that KAI1 expression significantly suppressed the metastatic potential of KAI1-transfected LoVo cells. CONCLUSION: Our results suggest that KAI1 might function as a negative regulator of colorectal carcinoma metastasis.