目的 :研究WT1基因在造血系统恶性肿瘤中的表达。方法 :采用逆转录酶链反应 (RT -PCR)方法 ,测定158例血液系统恶性肿瘤患者及30例正常对照者WT1基因的表达。结果 :急性白血病 (AL)WT1基因阳性表达率为45.3 %。慢性粒细胞白血病 (CML)慢性期及加速期无WT1基因表达 ,而急变期WT1基因表达阳性。骨髓增生异常综合征 (MDS)WT1的阳性表达率为22.4 %。淋巴瘤及恶性组织细胞病WT1的阳性表达率分别为39.1 %和41.7 %。而正常人无WT1基因的表达。结论 :WT1基因在造血系统恶性肿瘤患者中高表达。随着疾病的进展 ,WT1阳性表达率增高 Objective: To investigate the expression of WT1 gene in hematopoietic malignancies. Methods: The expression of WT1 gene in 158 patients with hematological malignancies and 30 normal controls was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). Results: The positive rate of WT1 gene in acute leukemia (AL) was 45.3%. The chronic and accelerated chronic myeloid leukemia (CML) WT1 gene expression, and blast crisis WT1 gene expression. The positive rate of WT1 in myelodysplastic syndrome (MDS) was 22.4%. The positive rates of WT1 in lymphomas and malignant histiocytosis were 39.1% and 41.7%, respectively. Normal WT1 gene expression. Conclusion: WT1 gene is highly expressed in patients with hematopoietic malignancies. With the progress of the disease, WT1 positive expression rate increased