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目的通过观察不同葡萄糖浓度下格列喹酮(Glq)刺激HIT-T15细胞分泌胰岛素的作用,了解Glq对β细胞的刺激模式。方法 HIT-T15细胞孵育于5、10、15mmol/L葡萄糖浓度的培养基中,8小时后加入Glq、格列本脲(Glb)、格列齐特(Glc),分别在不同时间点收集培养液,测定药物刺激前后的胰岛素分泌水平。结果在三种糖浓度下,Glq刺激的胰岛素分泌高峰均在加药后10分钟,在30分钟时又有一稍低的第二峰,随后逐渐下降,至180分钟后分泌速率为基础值的1.1～1.2倍;Glb呈单相分泌峰,峰值出现在45分钟,随后分泌速率逐渐下降,至180分钟后仍有基础值的1.5倍;在10 mmol/L糖浓度组,Glq刺激的胰岛素浓度时间曲线下面积(AUC)更小。Glc的分泌曲线类似Glq。结论 Glq与Glb对β细胞的刺激作用模式不同,前者较后者胰岛素分泌达峰时间更快、作用时间更短,AUC更小。
OBJECTIVE: To observe the effect of Glq on β-cell stimulation by observing the effect of Gliquidone (Glq) on insulin secretion in HIT-T15 cells under different glucose concentrations. Methods HIT-T15 cells were incubated in medium with glucose concentration of 5, 10 and 15 mmol / L. After 8 hours, Glq, glibenclamide (Glb) and Glicaine (Glc) were added and collected at different time points Liquid, determination of insulin secretion before and after drug stimulation level. Results At all three sugar concentrations, Glq-stimulated insulin secretion peaked at 10 minutes after dosing, with a slightly lower second peak at 30 minutes, and then decreased gradually to 1.1 after 1.1 minutes at a basal level of 1.1 ~ 1.2 times; Glb single-phase secretion peak, the peak appeared in 45 minutes, followed by a gradual decline in secretion rate, to 180 minutes after the baseline value is still 1.5 times; 10 mmol / L glucose concentration group, Glq-stimulated insulin concentration time Area under the curve (AUC) is smaller. Glc secretion curve similar to Glq. Conclusion Glq and Glb stimulate the β cell in different modes, the former is faster than the latter in peak insulin secretion, the action time is shorter and the AUC is smaller.