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一、高中生物(必修)实验一“生物组织中还原糖、脂肪、蛋白质的鉴定”做还原性糖的鉴定实验时,教材中要求斐林试剂的使用必须是将甲液和乙液先混合均匀后使用,不得分别加入生物组织样液进行检测,但经过实验证明:斐林试剂甲液0.1g/mL NaOH 溶液2 mL 和乙液0.05g/mL CuSO_4溶液4滴,无论是先混合马上使用还是两液依次加入试管后使用都有同样效果,且甲、乙两液不宜混合时间过长,对乙液滴数要求也不是很严格。做蛋白质鉴定实验时双缩脲试剂的使用 A 液0.1g/mLNaOH 溶液2 mL 和 B 液0.01 g/mL CuSO_4溶液3～4滴分
I. High school biology (required) Experiment 1 “Identification of Reducing Sugars, Fats, and Proteins in Biological Tissues” When conducting the identification experiment of reducing sugars, the textbook requires that the use of Filin’s reagent must be done by mixing the first liquid and the second liquid uniformly. After use, they should not be added to the biological tissue sample solution for testing. However, after experiments, it has been proved that: Feline Reagent A liquid 0.1g/mL NaOH solution 2mL and B solution 0.05g/mL CuSO_4 solution 4 drops, whether mixed or used immediately The two liquids are added to the test tube in turn to have the same effect, and the two liquids A and B are not suitable to be mixed for a long time. The requirement for the number of B liquid drops is not very strict. The use of biuret reagent for protein identification experiments A liquid 0.1 g/mL NaOH solution 2 mL and B solution 0.01 g/mL CuSO 4 solution 3 to 4 drops