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本文评价了伊达比星(idarubicin)对DNA合成、c-myc表达及MCF-7乳腺癌细胞生长的作用。进一步证明了在不存在DNA断裂和凋亡现象时乳腺癌细胞也能发生死亡。采用MTT四唑蓝染色试验来评价DNA生长抑制;采用碱洗脱方法来检测诱导DNA链断裂;对DNA合成的抑制是通过测量掺入DNA的标记胸
This article evaluates the effects of idarubicin on DNA synthesis, c-myc expression and MCF-7 breast cancer cell growth. Further evidence that breast cancer cells can die in the absence of DNA fragmentation and apoptosis. MTT assay was used to evaluate DNA growth inhibition; alkaline elution was used to detect induced DNA strand breaks; inhibition of DNA synthesis was measured by measuring DNA incorporation