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目的探讨人乳头瘤病毒(HPV)16/18在宫颈癌组织中的表达及其临床意义。方法采用PCR扩增+膜杂交法检测宫颈癌组织(A组,71例)、宫颈上皮内瘤变(CIN,B组,69例)和正常宫颈组织(C组,50例)HPV16/18的表达。结果A、B组HPV16/18阳性率分别为73.24%和24.24%,均高于C组的12.00%(P<0.05),A组高于B组(P<0.05)。A组CINⅢ级患者HPV16/18阳性率高于Ⅰ-Ⅱ级(P<0.05)。结论宫颈癌组织中HPV16/18阳性表达率高于CIN及正常宫颈组织;对宫颈病变检查者行常规的HPV16/18检测和对怀疑为CIN患者进行病理活检有助于宫颈恶性病变的早期诊断和治疗。
Objective To investigate the expression of human papillomavirus (HPV) 16/18 in cervical cancer and its clinical significance. Methods Cervical carcinoma tissues (A group, 71 cases), cervical intraepithelial neoplasia (CIN, B group, 69 cases) and normal cervical tissues (C group, 50 cases) were detected by PCR and membrane hybridization. expression. Results The positive rates of HPV16 / 18 in group A and group B were 73.24% and 24.24%, respectively, which were higher than those in group C (12.00%, P <0.05). The positive rate of HPV16 / 18 in group CINⅢ was higher than that in group Ⅰ-Ⅱ (P <0.05). Conclusion The positive rate of HPV16 / 18 in cervical cancer is higher than that in CIN and normal cervical tissues. The routine HPV16 / 18 test for cervical lesions and pathological biopsy in suspected CIN patients are helpful for the early diagnosis of cervical malignant lesions. treatment.