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以中江县患病丹参根际与非根际土壤、正常生长丹参根际与非根际土壤为研究对象,在测定土壤理化性质基础上,采用PCR-DGGE技术研究了土壤真菌类群多样性。结果表明,与正常生长丹参土壤相比,患病丹参土壤理化性质发生了较大变化,其中,土壤有机质、全氮、碱解氮、速效磷和速效钾含量分别降低了60%、52%、18%、18%和45%。PCR-DGGE分析表明,连作导致患病丹参根际与非根际土壤真菌类群差异变小;对代表性条带克隆测序,结果表明Acremonium sclerotigenum,茎点霉属(Phoma sp.),Phialocephala fortinii和Chaetomium sp.为土壤中优势类群。说明土壤肥力退化及致病真菌是造成丹参连作障碍的原因。
The rhizosphere and non-rhizosphere soil of Salvia miltiorrhiza in Zhongjiang county, the rhizosphere and non-rhizosphere soil of normal growth Salvia miltiorrhiza were studied. Based on the determination of soil physical and chemical properties, PCR-DGGE was used to study the diversity of soil fungi. The results showed that compared with the normal growth of Salvia miltiorrhiza soil, the soil physico-chemical properties of Salvia miltiorrhiza had a great change, in which the content of soil organic matter, total nitrogen, available nitrogen, available phosphorus and available potassium decreased by 60%, 52% 18%, 18% and 45%. PCR-DGGE analysis showed that there was a small difference in fungal community between rhizosphere soil and non-rhizosphere soil of continuous cropping. The representative bands were cloned and sequenced. The results showed that Acremonium sclerotigenum, Phoma sp., Phialocephala fortinii and Chaetomium sp. Is the dominant group in soils. Explain soil fertility degradation and pathogenic fungi cause Danshen continuous obstacle.