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目的采用HPLC测定赤胫散中的3,3’-二甲基鞣花酸和3,3’,4’-三甲基鞣花酸。方法色谱柱为Dikma-C18柱,流动相为甲醇-乙腈-0.1%磷酸(17∶33∶50),流速1 m L·min-1,检测波长248 nm,柱温30℃。结果 3,3’-二甲基鞣花酸0.198~0.990μg(r=0.9991)、3,3’,4’-三甲基鞣花酸0.098~0.491μg(r=0.9990)与峰面积的线性关系良好,回收率分别为102.40%、98.41%,RSD分别为2.25%、1.87%(n=6)。结论所用方法简便、快捷、准确,可用于赤胫散药材的质量控制。
Objective To determine the content of 3,3’-dimethyl-ellagic acid and 3,3 ’, 4’-trimethyl-ellagic acid in Chis-chikan San by HPLC. Methods The column was Dikma-C18. The mobile phase consisted of methanol-acetonitrile-0.1% phosphoric acid (17:33:50) and the flow rate was 1 m L · min-1. The detection wavelength was 248 nm and the column temperature was 30 ℃. Results The linear range of the linearity of peak area was 0.198 ~ 0.990μg (r = 0.9991) for 3,3’-dimethyl ellagic acid and 0.098 ~ 0.491μg for 3,3 ’, 4’-trimethyl ellagic acid The results showed that the recovery rates were 102.40% and 98.41% respectively, with RSDs of 2.25% and 1.87%, respectively (n = 6). Conclusion The method is simple, rapid and accurate, and can be used for the quality control of red tibia medicinal herbs.