论文部分内容阅读
云南种植的亚麻,其木质素含量比在湖南种植的显著升高。采用基因芯片技术,对湖南沅江和云南昆明栽培的亚麻茎皮组织基因差异表达进行了初步分析。以湖南沅江样品为对照,在9600个cDNA微矩阵点中,云南昆明样品有效差异表达Ratio值I≥2或≤0.5的cDNA序列共2051个,其中上调表达序列674个,下调表达序列1378个。按功能分类,这些差异表达序列主要为细胞结构,其次是能量和蛋白合成。表达差异在8倍以上的序列216个,其中已知功能序列76个,占总数的35.2%。这些高度差异表达序列中,有一些如几丁质酶、MYB转录因子R3是植物细胞壁合成过程的重要基因。
Flaxseeds grown in Yunnan Province have significantly higher lignin content than those grown in Hunan. Gene chip technology was used to analyze the differential gene expression of flax bark tissue cultivated in Yuanjiang, Hunan Province and Kunming, Yunnan Province. Among the 9600 cDNA microarray spots, 2051 cDNA sequences were efficiently differentially expressed in Kunming samples with I≥2 or ≤0.5, of which 674 were up-regulated and 1378 were down-regulated. By functional classification, these differentially expressed sequences are mainly cellular structures, followed by energy and protein synthesis. There were 216 sequences with more than 8-fold difference in expression, of which 76 were known, occupying 35.2% of the total. Some of these highly differentially expressed sequences, such as chitinase, MYB transcription factor R3 is an important gene in plant cell wall synthesis.